TNF-beta/TNFSF1 蛋白, Human (HEK293, His)

TNF-β protein acts as a homotrimeric cytokine that binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR, and TNFRSF14/HVEM. TNF-beta/TNFSF1 Protein, Human (HEK293, His) is the recombinant human-derived TNF-beta/TNFSF1 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of TNF-beta/TNFSF1 Protein, Human (HEK293, His) is 171 a.a., with molecular weight of 20.8 kDa.

多项研究表明，同源三聚体结构的 TNF-β 蛋白表现出与 TNFRSF1A/TNFR1、TNFRSF1B/TNFBR 和 TNFRSF14/HVEM 的结合能力。此外，在与 LTB 的异三聚体关联中，它与 TNFRSF3/LTBR 形成相互作用，强调了其不同的受体参与。这种细胞因子主要由淋巴细胞产生，在体外和体内均表现出针对多种肿瘤细胞的细胞毒性作用。在结构上，TNF-β以同源三聚体和异源三聚体形式存在，后者包含两个LTB和一个LTA亚基，或者在较小程度上包含两个LTA和一个LTB亚基。此外，TNF-β 与 TNFRSF14 相互作用，强调其在介导复杂免疫反应中的关键作用。

Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 μg/mL can bind human TNFRSF1B, the EC₅₀ is ≤4 ng/mL.

Human

HEK293

N-6*His

P01374 (L35-L205)

4049  [NCBI]

LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL

20.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.