M-CSF 蛋白, Human (223a.a, HEK293, His)

The M-CSF protein is an important cytokine that modulates hematopoietic precursor cells, especially mononuclear phagocytes, affecting innate immunity and inflammation through the release of proinflammatory chemokines. It is essential for osteoclast proliferation and regulates bone resorption, bone development and fertility. M-CSF Protein, Human (223a.a, HEK293, His) is the recombinant human-derived M-CSF protein, expressed by HEK293 , with C-6*His labeled tag. The total length of M-CSF Protein, Human (223a.a, HEK293, His) is 223 a.a., with molecular weight of ~41.0 kDa.

M-CSF 蛋白是一种重要的细胞因子，参与调节造血前体细胞（特别是巨噬细胞和单核细胞等单核吞噬细胞）的存活、增殖和分化。它通过促进促炎趋化因子的释放在先天免疫和炎症过程中发挥至关重要的作用。此外，M-CSF 蛋白对于破骨细胞增殖和分化、调节骨吸收和正常骨骼发育至关重要。它也是正常男性和女性生育能力所必需的。此外，M-CSF 蛋白有助于肌动蛋白细胞骨架的重组，促进膜皱褶形成、细胞粘附和细胞迁移。此外，它在脂蛋白清除中发挥作用。M-CSF 蛋白可以不同的形式存在，例如同二聚体或异二聚体构型，并且它与 CSF1R 相互作用。

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 1.323 ng/mL, corresponding to a specific activity is 7.559×10⁵ units/mg.

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  Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 1.323 ng/mL, corresponding to a specific activity is 7.559×10⁵ units/mg.

Human

HEK293

C-6*His

P09603 (E33-R255)

1435  [NCBI]

EEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCNCLYPKAIPSSDPASVSPHQPLAPSMAPVAGLTWEDSEGTEGSSLLPGEQPLHTVDPGSAKQRPPR

Approximately 41.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<0.01 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.