1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. MINPP1 蛋白, Human (HEK293, His)

MINPP1 蛋白, Human (HEK293, His)

目录号: HY-P70937
产品使用指南

MINPP1 蛋白是一种双功能磷酸酶,通过磷酸肌醇 5- 和磷酸肌醇 6- 磷酸酶活性调节肌醇五磷酸 (InsP5) 和肌醇六磷酸 (InsP6) 水平。它还充当 2,3-二磷酸甘油酸 3-磷酸酶,对于骨骼发育和软骨细胞转变至关重要。通过控制细胞内肌醇多磷酸,MINPP1 可能影响阳离子稳态,通过游离阳离子可用性影响神经细胞信号传导。MINPP1 蛋白, Human (HEK293, His) 是重组的 MINPP1 蛋白,由 HEK293 表达,带有 C-6*His 标签。MINPP1 蛋白, Human (HEK293, His) 全长 457 个氨基酸,分子量约为 ~56.0 kDa。

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描述

MINPP1 Protein, a dual-function phosphatase, regulates inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6) levels through phosphoinositide 5- and phosphoinositide 6-phosphatase activity. It also acts as a 2,3-bisphosphoglycerate 3-phosphatase, crucial for bone development and chondrocyte transition. By controlling intracellular inositol polyphosphates, MINPP1 potentially influences cation homeostasis, impacting neural cell signaling through free cation availability. MINPP1 Protein, Human (HEK293, His) is the recombinant human-derived MINPP1 protein, expressed by HEK293, with C-6*His labeled tag. The total length of MINPP1 Protein, Human (HEK293, His) is 457 a.a., with molecular weight of ~56.0 kDa.

研究背景

MINPP1 蛋白充当双功能磷酸酶,表现出磷酸肌醇 5- 和磷酸肌醇 6- 磷酸酶活性,可调节肌醇五磷酸 (InsP5) 和肌醇六磷酸 (InsP6) 的细胞水平。此外,它还充当 2,3-二磷酸甘油酸 3-磷酸酶,催化 2,3-二磷酸甘油酸 (2,3-BPG) 去磷酸化,产生磷酸-D-甘油酸,而不形成 3-磷酸甘油酸。这些活动对于细胞过程至关重要,包括骨发育,特别是软骨内骨化,并且可能有助于软骨细胞从增殖到肥大的转变。通过调节细胞内肌醇多磷酸,MINPP1 在控制细胞内阳离子稳态方面发挥潜在作用,影响神经细胞信号传导所需的游离阳离子的可用性。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9UNW1 (S31-L487)

基因 ID
蛋白结构
N-term
MINPP1 (S31-L487)
Accession # Q9UNW1
6*His
C-term
同用名
Multiple Inositol Polyphosphate Phosphatase 1; 2,3-Bisphosphoglycerate 3-Phosphatase; 2,3-BPG Phosphatase; Inositol (1,3,4,5)-Tetrakisphosphate 3-Phosphatase; Ins(1,3,4,5)P(4) 3-Phosphatase; MINPP1; MIPP
氨基酸序列

SLLEPRDPVASSLSPYFGTKTRYEDVNPVLLSGPEAPWRDPELLEGTCTPVQLVALIRHGTRYPTVKQIRKLRQLHGLLQARGSRDGGASSTGSRDLGAALADWPLWYADWMDGQLVEKGRQDMRQLALRLASLFPALFSRENYGRLRLITSSKHRCMDSSAAFLQGLWQHYHPGLPPPDVADMEFGPPTVNDKLMRFFDHCEKFLTEVEKNATALYHVEAFKTGPEMQNILKKVAATLQVPVNDLNADLIQVAFFTCSFDLAIKGVKSPWCDVFDIDDAKVLEYLNDLKQYWKRGYGYTINSRSSCTLFQDIFQHLDKAVEQKQRSQPISSPVILQFGHAETLLPLLSLMGYFKDKEPLTAYNYKKQMHRKFRSGLIVPYASNLIFVLYHCENAKTPKEQFRVQMLLNEKVLPLAYSQETVSFYEDLKNHYKDILQSCQTSEECELARANSTSDEL

分子量

Approximately 56.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MINPP1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MINPP1 Protein, Human (HEK293, His)
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HY-P70937
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