1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. MINPP1 蛋白, Human (HEK293, His)

MINPP1 蛋白, Human (HEK293, His)

目录号: HY-P70937
产品使用指南 技术支持

MINPP1 蛋白是一种双功能磷酸酶,可通过磷酸肌醇 5- 和磷酸肌醇 6- 磷酸酶活性调节肌醇五磷酸 (InsP5) 和植酸六磷酸 (InsP6) 水平。它还充当 2,3-二磷酸甘油酸 3-磷酸酶,对于骨骼发育和软骨细胞转化至关重要。MINPP1 蛋白, Human (HEK293, His) 是重组的 MINPP1 蛋白,由 HEK293 表达,带有 C-6*His 标签。MINPP1 蛋白, Human (HEK293, His) 全长 457 个氨基酸,分子量约为 ~56.0 kDa。

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描述

The MINPP1 protein is a bifunctional phosphatase that regulates inositol pentaphosphate (InsP5) and phytate hexaphosphate (InsP6) levels through phosphoinositide 5- and phosphoinositide 6-phosphatase activities. It also acts as 2,3-bisphosphoglycerate 3-phosphatase, which is essential for bone development and chondrocyte transformation. MINPP1 Protein, Human (HEK293, His) is the recombinant human-derived MINPP1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MINPP1 Protein, Human (HEK293, His) is 457 a.a., with molecular weight of ~56.0 kDa.

研究背景

MINPP1 蛋白充当双功能磷酸酶,表现出磷酸肌醇 5- 和磷酸肌醇 6- 磷酸酶活性,可调节肌醇五磷酸 (InsP5) 和肌醇六磷酸 (InsP6) 的细胞水平。此外,它还充当 2,3-二磷酸甘油酸 3-磷酸酶,催化 2,3-二磷酸甘油酸 (2,3-BPG) 去磷酸化,产生磷酸-D-甘油酸,而不形成 3-磷酸甘油酸。这些活动对于细胞过程至关重要,包括骨发育,特别是软骨内骨化,并且可能有助于软骨细胞从增殖到肥大的转变。通过调节细胞内肌醇多磷酸,MINPP1 在控制细胞内阳离子稳态方面发挥潜在作用,影响神经细胞信号传导所需的游离阳离子的可用性。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9UNW1 (S31-L487)

基因 ID
蛋白结构
N-term
MINPP1 (S31-L487)
Accession # Q9UNW1
6*His
C-term
中文名
MINPP1 蛋白
同用名
Multiple Inositol Polyphosphate Phosphatase 1; 2,3-Bisphosphoglycerate 3-Phosphatase; 2,3-BPG Phosphatase; Inositol (1,3,4,5)-Tetrakisphosphate 3-Phosphatase; Ins(1,3,4,5)P(4) 3-Phosphatase; MINPP1; MIPP
氨基酸序列

SLLEPRDPVASSLSPYFGTKTRYEDVNPVLLSGPEAPWRDPELLEGTCTPVQLVALIRHGTRYPTVKQIRKLRQLHGLLQARGSRDGGASSTGSRDLGAALADWPLWYADWMDGQLVEKGRQDMRQLALRLASLFPALFSRENYGRLRLITSSKHRCMDSSAAFLQGLWQHYHPGLPPPDVADMEFGPPTVNDKLMRFFDHCEKFLTEVEKNATALYHVEAFKTGPEMQNILKKVAATLQVPVNDLNADLIQVAFFTCSFDLAIKGVKSPWCDVFDIDDAKVLEYLNDLKQYWKRGYGYTINSRSSCTLFQDIFQHLDKAVEQKQRSQPISSPVILQFGHAETLLPLLSLMGYFKDKEPLTAYNYKKQMHRKFRSGLIVPYASNLIFVLYHCENAKTPKEQFRVQMLLNEKVLPLAYSQETVSFYEDLKNHYKDILQSCQTSEECELARANSTSDEL

分子量

Approximately 56.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MINPP1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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