MUSK 蛋白, Mouse (Myc, His-SUMO)

MUSK protein is a receptor tyrosine kinase that plays a critical role in the formation of the neuromuscular junction (NMJ).MUSK is activated by the AGRIN-LRP4 complex and regulates the NMJ through gene expression, actin cytoskeleton reorganization, and acetylcholine receptor (AChR) clustering.MUSK Protein, Mouse (Myc, His-SUMO) is the recombinant mouse-derived MUSK protein, expressed by E.coli , with C-Myc, N-SUMO, N-10*His labeled tag.

MUSK 蛋白是一种受体酪氨酸激酶，在神经肌肉接头 (NMJ)（连接运动神经元和骨骼肌的突触）的形成和维护中发挥着至关重要的作用。LRP4 通过招募 AGRIN 来激活 MUSK 信号复合物，从而导致 MUSK 的磷酸化和激活。在肌管中，MUSK 的激活通过控制多个过程来调节 NMJ 的形成，包括突触下核中特定基因的表达、肌动蛋白细胞骨架的重组以及突触后膜中乙酰胆碱受体 (AChR) 的聚集。AChR 磷酸化和聚类的这种调节是由 ABL1 和 Src 家族激酶的激活介导的，而 ABL1 和 Src 家族激酶又反过来调节 MUSK。此外，MUSK 与 DVL1 和 PAK1 形成三元复合物，这对于 MUSK 依赖性的 AChR 聚类调节非常重要。MUSK 还可以通过 FNTA 正向调节 Rho 家族 GTPases，介导 RAC1（肌动蛋白细胞骨架和基因表达的关键调节因子）的磷酸化和激活。MUSK 信号传导的下游效应器包括 DNAJA3，其在 MUSK 下游发挥作用。此外，麝香还可能通过介导胆碱能反应、突触可塑性和记忆形成在中枢神经系统中发挥作用。

Measured by its binding ability in a functional ELISA. Recombinant Mouse MuSK binds to Recombinant Human LRP-4 and Agrin. The ED₅₀ for this effect is 0.4847 μg/mL.

Mouse

E. coli

C-Myc;N-SUMO;N-10*His

Q61006 (E22-S494)

18198  [NCBI]

EKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCIANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDNALRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEVFARILRAPESHNVTFGSFVTLRCTAIGIPVPTISWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATVSIAEWSKSQKDSQGYCAQYRGEVCDAVLAKDALVFFNTSYRDPEDAQELLIHTAWNELKAVSPLCRPAAEALLCNHLFQECSPGVVPTPMPICREYCLAVKELFCAKEWQAMEGKAHRGLYRSGMHLLPVPECSKLPSMHRDPTACTRLPYLDYKKENITTFPSITS

Approximately 69.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.