PCSK9 蛋白, Human (D374Y, HEK293, His)

PCSK9 protein, a crucial regulator of plasma cholesterol homeostasis, influences LDL receptor family members like LDLR and VLDLR. It facilitates their degradation within intracellular compartments, enhancing hepatic LDLR degradation through a non-proteolytic mechanism. PCSK9 also exhibits LDLR-independent inhibition of APOB degradation and regulates ENaC-mediated Na(+) absorption, influencing neuronal apoptosis. Notably, it modulates LRP8/APOER2 levels and associated anti-apoptotic pathways. PCSK9 Protein, Human (D374Y, HEK293, His) is the recombinant human-derived PCSK9 protein, expressed by HEK293 , with C-10*His labeled tag and D374Y mutation. The total length of PCSK9 Protein, Human (D374Y, HEK293, His) is 662 a.a., with molecular weight of ~17 & 70 kDa, respectively.

PCSK9 蛋白在血浆胆固醇稳态的复杂协调中发挥着关键调节作用。证明其对低密度脂蛋白受体家族成员的影响，包括低密度脂蛋白受体 (LDLR)、极低密度脂蛋白受体 (VLDLR)、载脂蛋白 E 受体 (LRP1/APOER) 和载脂蛋白受体 2 (LRP8/APOER2) ），PCSK9 促进它们在细胞内酸性区室中的降解。它采用非蛋白水解机制，通过网格蛋白 LDLRAP1/ARH 介导的途径增强肝脏 LDLR 降解，可能阻碍 LDLR 回收并将其引导至溶酶体降解。此外，PCSK9 通过自噬体/溶酶体途径表现出 LDLR 独立的 APOB 细胞内降解抑制作用，并在早期分泌途径中非乙酰化 BACE1 中间体的处理中发挥作用。值得注意的是，它通过增强 ENaC 蛋白酶体降解来调节上皮 Na(+) 通道 (ENaC) 介导的 Na(+) 吸收，并通过调节 LRP8/APOER2 水平和相关的抗凋亡信号通路影响神经元凋亡。

Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED₅₀ for this effect is 16.92 ng/mL, corresponding to a specificactivity is 5.91×10⁴ Unit/mg.

• 
  Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED₅₀ for this effect is 16.92 ng/mL, corresponding to a specificactivity is 5.91×10⁴ Unit/mg.

Human

HEK293

C-10*His

Q8NBP7 (Q31-Q692, D374Y)

255738  [NCBI]

QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSYCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQ

Approximately 17 & 70 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.