Progranulin/PGRN 蛋白, Mouse (HEK293, His)

Progranulin (PGRN) proteins regulate lysosomal function, affecting protein transport, enzymatic activity, and acidification. It degrades mature cathepsin D via cathepsin B. Progranulin/PGRN Protein, Mouse (572a.a, HEK293, His) is the recombinant mouse-derived Progranulin/PGRN protein, expressed by HEK293 , with C-His labeled tag. The total length of Progranulin/PGRN Protein, Mouse (572a.a, HEK293, His) is 572 a.a., with molecular weight of 70-90 kDa.

颗粒体蛋白前体 (PGRN) 蛋白是溶酶体功能的重要调节剂和生长因子，其作用涵盖炎症、伤口愈合和细胞增殖。PGRN 协调蛋白质向溶酶体的运输，影响溶酶体酶活性，并促进溶酶体酸化，最终导致组织蛋白酶 B 降解成熟的组织蛋白酶 D。除了其溶酶体功能外，PGRN 还充当伤口愈合中的生长因子，直接影响真皮成纤维细胞和内皮细胞细胞，促进细胞分裂、迁移和毛细血管样小管结构的形成。它通过阻止 TNF 介导的中性粒细胞活化、防止氧化剂和蛋白酶的释放，进一步促进上皮细胞增殖。此外，PGRN 在调节神经元炎症、保护神经元存活、轴突生长和整体神经元完整性方面发挥着关键作用。然而，它对上皮细胞表现出相反的作用，抑制其增殖并促进 IL-8 的分泌。PGRN 的多方面作用凸显了其在细胞过程中的多功能功能，对各种生理和病理条件都有影响。

Measured by its ability to inhibit THP-1 human acute monocyte migration in the presence of 10 ng/mL MCP-1.

Mouse

HEK293

C-His

P28798 (T18-L589)

14824  [NCBI]

TQCPDGQFCPVACCLDQGGANYSCCNPLLDTWPRITSHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDPVPRPLL

70-90 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.