Prostatic acid phosphatase/ACPP 蛋白, Human (HEK293, His)

Acid prostatic phosphatase (ACPP) is an enzyme that catalyzes the conversion of orthophosphate monoesters into alcohol and orthophosphate. ACPP is regulated by androgens and secreted by prostate epithelial cells. Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is the recombinant human-derived Prostatic acid phosphatase/ACPP protein, expressed by HEK293 , with C-His labeled tag. The total length of Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is 350 a.a., with molecular weight of 43-55 kDa.

前列腺酸性磷酸酶（ACPP）是一种负责催化正磷酸单酯转化为酒精和正磷酸盐的酶。ACPP 在雄激素调节下合成，由前列腺上皮细胞分泌。已经鉴定出一种选择性剪接的转录物变体，编码更长的亚型，其跨膜结构域定位于质膜-内体-溶酶体途径。ACPP 的表达特别局限于前列腺，强调了其作为与该特定组织相关的标记物的作用。

1. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >100,000 pmol/min/μg.
2. Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). which can be measured by absorbance at 410 nm. The specific activity is 4.264×10⁷ pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His

NP_001127666.1 (K33-K382)

55  [NCBI]

MRAAPLLLARAASLSLGFLFLLFFWLDRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQVLK

43-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized a 0.22 μm filtered solution of 20 mM PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.