SCF 蛋白, Mouse (HEK293, His)

SCF proteins are ligands for the KIT receptor-type protein tyrosine kinase and are critical for multiple cellular processes, including survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis.Binding to KIT activates multiple signaling pathways involving PIK3R1, AKT1, GRB2, RAS, RAF1, MAP kinase, STAT family members and PLCG1, producing signaling molecules.SCF Protein, Mouse (HEK293, His) is the recombinant mouse-derived SCF protein, expressed by HEK293 , with C-6*His labeled tag.

干细胞因子（SCF）蛋白作为受体型蛋白酪氨酸激酶KIT的配体，在多种细胞过程的调节中发挥着至关重要的作用。其功能包括控制细胞存活和增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移和黑素生成。与 KIT 结合后，SCF 激活多种信号通路，包括 PIK3R1 的磷酸化和随后 AKT1 的激活。这种相互作用还会触发涉及 GRB2、RAS、RAF1 和 MAP 激酶 MAPK1/ERK2 和/或 MAPK3/ERK1 的信号级联。此外，SCF 和 KIT 促进 STAT 家族成员（STAT1、STAT3 和 STAT5）以及 PLCG1 的激活，从而产生二酰基甘油和肌醇 1,4,5-三磷酸作为细胞信号分子。SCF 与其他细胞因子（可能是白细胞介素）协同作用，与 KIT 形成非共价连接的同二聚体和异四聚体，促进 KIT 二聚化并随后通过自磷酸化激活。

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 2.879 ng/mL, corresponding to a specific activity is 3.47×10⁵ units/mg.

Mouse

HEK293

C-6*His

P20826 (K26-A189)

17311  [NCBI]

KEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENAPKNIKESPKRPETRSFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVA

19-35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.