SNAP-alpha 蛋白, Human (His)

SNAP-α proteins are critical in vesicle trafficking, mediating dynamic exchange between the endoplasmic reticulum and the Golgi apparatus. It cooperates with GNA12 to localize CDH5 to the plasma membrane. SNAP-alpha Protein, Human (His) is the recombinant human-derived SNAP-alpha protein, expressed by E. coli , with N-6*His labeled tag. The total length of SNAP-alpha Protein, Human (His) is 295 a.a., with molecular weight of 33-35 kDa.

SNAP-α 蛋白是囊泡运输中不可或缺的参与者，促进内质网和高尔基体之间的动态交换。它与 GNA12 的合作扩大了其影响力，促进了 CDH5 定位到质膜。此外，SNAP-α参与复杂的蛋白质相互作用，与PRKCABP形成复合物并参与破坏GRIA2和PRKCABP之间的相互作用，从而驱动GRIA2的内化。在复杂的类似 SNARE 的组装中，SNAP-alpha 与 ZW10、USE1L、RINT1、STX18 和 NAPA 并列，揭示了其在膜融合事件中不可或缺的作用。这种多功能蛋白质通过与 VAMP8、VTI1A 和 STX12 结合，进一步扩展了其相互作用库。SNAP-alpha 在多种细胞过程中复杂的相互作用强调了它作为细胞内运输和膜动力学复杂景观中关键协调者的重要性。

Human

E. coli

N-6*His

P54920 (M1-R295)

8775  [NCBI]

MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR

33-35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of sterile 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01%Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.