Latent TGF beta 1/TGFB1 蛋白, Rat (HEK293, His)

Latent TGF beta 1 (latent TGFB1) is the inactive form of TGF-B1. Latent TGF beta 1 associates with the extracellular matrix (ECM) via LTBP. LTBPs are components of the ECM, so that the proteolytic cleavage of LTBP can lead to the release of latent TGF-beta 1 from the matrix. Besides, the proteolytic cleavage and liberation of active TGFB1 is performed by BMP-1, by a variety of matrix metalloproteinases (MMPs)^[1][3]. Latent TGF beta 1/TGFB1 Protein, Rat (HEK293, His) is the recombinant rat-derived Latent TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Latent TGF beta 1/TGFB1 Protein, Rat (HEK293, His) is 361 a.a., with molecular weight of ~55 & 38 & 16 kDa, respectively.

TGF-B1 通常以非活性、潜伏形式被分泌出来。潜伏 TGFβ1 (latent TGFB1) 由三个不同的成分组成：成熟的 TGFB1 (一种二硫键二聚体)、TGFB1 前体的 N 末端残余物、和一种新型蛋白质，称为潜伏 TGFB1 结合蛋白 (LTBP)^[2]。
尽管 latent TGF beta 1 可通过各种机制转化为其生物活性形式，但所有机制都涉及 TGFB1 从可溶性 SLC (小潜在复合物) 和/或 ECM 结合 LLC (大潜在复合物) 中的 LAP-B1 解离。此外，蛋白水解裂解是 latent TGFB1 激活的最重要的细胞机制。latent TGF beta 1 通过 LTBP 与细胞外基质 (ECM) 结合。LTBP 是 ECM 的组成部分，因此 LTBP 的蛋白水解裂解可导致潜在的 TGF-β 1 从基质中释放。此外，LLC 的蛋白水解和活性 TGFB1 的释放是由 BMP-1 和多种基质金属蛋白酶 (MMP) 介导的^[1][3] 。

Rat

HEK293

C-His

P17246 (L30-S390)

59086  [NCBI]

LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDIREAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 55&38&16 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.