TRIB3 蛋白, Human (sf9, GST)

TRIB3 protein is an inactive kinase that centrally regulates the integrated stress response (ISR) and inhibits DDIT3/CHOP transcriptional activity. It causes DDIT3/CHOP-dependent cell death during endoplasmic reticulum (ER) stress, particularly in programmed neuronal cell death. TRIB3 Protein, Human (sf9, GST) is the recombinant human-derived TRIB3 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of TRIB3 Protein, Human (sf9, GST) is 358 a.a., with molecular weight of ~65.8 kDa.

TRIB3 蛋白是一种非活性激酶，作为综合应激反应 (ISR) 的关键调节因子发挥作用，ISR 是适应不同应激条件的关键机制。它作为 DDIT3/CHOP 转录活性的抑制剂发挥作用，在内质网 (ER) 应激期间，特别是在程序性神经元细胞死亡的情况下，在内质网 (ER) 应激期间在内质网 (ER) 依赖性细胞死亡中发挥作用。TRIB3 作为 ISR 内的负反馈调节因子，与 ATF4 相互作用，抑制 ATF4 转录活性，同时受到 ATF4 表达的促进。此外，TRIB3 通过直接与 Akt 激酶结合来破坏胰岛素信号传导，阻碍其激活，并可能掩盖 AKT1 中的“Thr-308”磷酸化位点。此外，TRIB3 与各种信号分子相互作用，包括 NF-kappa-B 反式激活因子 p65 RELA、MAPK 激酶和 APOBEC3 蛋白，展示了其在不同细胞过程和应激反应中的多方面作用。

Human

Sf9 insect cells

N-GST

Q96RU7 (M1-G358)

57761  [NCBI]

Approximately 65.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM Reduced Glutathione, 10% glycerol, 0.5 mM PMSF.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.