TrkA 蛋白, Human (HEK293)

TrkA Protein, Human (HEK293) is the high affinity catalytic receptor for the neurotrophin, nerve growth factor (NGF), and mediates the multiple effects of NGF, which include neuronal differentiation and avoidance of programmed cell death.

酪氨酸激酶受体 A (TrkA) 是神经营养蛋白、神经生长因子 (NGF) 的高亲和力催化受体，介导 NGF 的多种作用，包括神经元分化和避免程序性细胞死亡。TrkA 最初是从结肠肿瘤中克隆出来的；癌症是通过易位发生的，易位导致 TrkA 激酶结构域^[1][2]激活。

The ED₅₀ is <1 μg/mL as measured by a neutralization assay in the presence of 10.0 ng/ml human β-NGF using TF-1 Cells.

Human

HEK293

Tag Free

P04629-1 (A34-P407)

4914  [NCBI]

APCPDACCPHGSSGLRCTRDGALDSLHHLPGAENLTELYIENQQHLQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTC

65-85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Benito-Gutiérrez E, et al. Origin and evolution of the Trk family of neurotrophic receptors. Mol Cell Neurosci. 2006 Feb;31(2):179-92.  [Content Brief]

  [2]. Lambiase A, et al. Molecular basis for keratoconus: lack of TrkA expression and its transcriptional repression by Sp3. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16795-800.  [Content Brief]