2. Apoptosis
  3. Apoptosis Caspase
  4. 28-Deoxonimbolide

28-Deoxonimbolide 是一种宁宾 (nimbin, HY-N3187) 型柠檬苦素，可以从 Azadirachta indica 种子提取物中分离出来。28-Deoxonimbolide 具有抗癌活性。 28-Deoxonimbolide 通过线粒体和死亡受体介导的途径诱导 HL60 细胞凋亡 (apoptosis)。

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28-Deoxonimbolide Chemical Structure

28-Deoxonimbolide Chemical Structure

CAS No. : 126005-94-5

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28-Deoxonimbolide 相关抗体

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

28-Deoxonimbolide is a nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways[1].

IC50 & Target

Caspase-8

 

Caspase-9

 

Caspase-3

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
9.3 μM
Compound: 28
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
AZ-521 cell line IC50
2.4 μM
Compound: 28
Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human AZ-521 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
BC1 cell line ED50
1.34 μg/mL
Compound: 2
Cytotoxicity against human BC1 cells after 72 hrs
Cytotoxicity against human BC1 cells after 72 hrs
[PMID: 2614419]
Col2 ED50
1.81 μg/mL
Compound: 2
Cytotoxicity against human Col2 cells after 72 hrs
Cytotoxicity against human Col2 cells after 72 hrs
[PMID: 2614419]
HL-60 IC50
2.7 μM
Compound: 28
Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
HT-1080 ED50
1.04 μg/mL
Compound: 2
Cytotoxicity against human HT1080 cells after 48 hrs
Cytotoxicity against human HT1080 cells after 48 hrs
[PMID: 2614419]
KB ED50
1.3 μg/mL
Compound: 2
Cytotoxicity against human KB cells after 72 hrs
Cytotoxicity against human KB cells after 72 hrs
[PMID: 2614419]
P388 ED50
0.66 μg/mL
Compound: 2
Cytotoxicity against mouse P388 cells after 48 hrs
Cytotoxicity against mouse P388 cells after 48 hrs
[PMID: 2614419]
SK-BR-3 IC50
1.7 μM
Compound: 28
Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human SK-BR-3 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 21381696]
SK-MEL-2 ED50
2.05 μg/mL
Compound: 2
Cytotoxicity against human SK-MEL-2 cells after 72 hrs
Cytotoxicity against human SK-MEL-2 cells after 72 hrs
[PMID: 2614419]
体外研究
(In Vitro)

28-Deoxonimbolide (1-100 μM，48 小时) 对 HL60 (白血病)、A549 (肺)、AZ521 (胃)、SK-BR-3 (乳腺)、CRL1579 (黑色素瘤)和 RPMI1788 (正常淋巴细胞) 显示强有力的细胞毒性[1]。
28-Deoxonimbolide (30 μM, 0-24 h) 诱导 HL60 细胞凋亡[1]。
28-Deoxonimbolide (30 μM, 0-24 h) 可增加裂解的 caspases-8、-9 和 -3 的水平[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

28-Deoxonimbolide 相关抗体:

Cell Cytotoxicity Assay[1]

Cell Line: HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788
Concentration: 1-100 μM
Incubation Time: 48 h
Result: Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC50 values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively.

Apoptosis Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control).

Western Blot Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3.
分子量

452.54

Formula

C27H32O6
CAS 号
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

28-Deoxonimbolide 相关分类

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Keywords:

28-Deoxonimbolide126005-94-5ApoptosisCaspaseHL60A549AZ521SK-BR-3CRL1579 cellsAzadirachta indicaInhibitorinhibitorinhibit

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