2. Cell Cycle/DNA Damage Epigenetics PI3K/Akt/mTOR
  3. HDAC mTOR
  4. Pomiferin

Pomiferin  (Synonyms: 橙桑黄酮; NSC 5113)

目录号: HY-N4315 纯度: 98.98%
COA 产品使用指南 技术支持

Pomiferin (NSC 5113) 为 HDAC 和 mTOR 的抑制剂，IC50 值分别为 1.05 μM 和 6.2 µM。

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Pomiferin Chemical Structure

Pomiferin Chemical Structure

CAS No. : 572-03-2

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规格 价格 是否有货 数量
1 mg ￥2100
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5 mg ￥5320
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Customer Review

Pomiferin 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 HDAC 亚型特异性产品:

查看所有亚型
HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

查看 mTOR 亚型特异性产品:

查看所有亚型
mTOR mTORC1 mTORC2

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Pomiferin (NSC 5113) acts as an potential inhibitor of HDAC, with an IC50 of 1.05 μM, and also potently inhibits mTOR (IC50, 6.2 µM).

IC50 & Target[1][2]

HDAC

1.05 μM (IC50)

mTOR

6.2 μM (IC50)

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
ACHN GI50
3.18 μM
Compound: 2, Pomiferin
Growth inhibition of human ACHN cells after 21 hrs by MTT assay
Growth inhibition of human ACHN cells after 21 hrs by MTT assay
[PMID: 17662606]
HCT-15 GI50
1.32 μM
Compound: 2, Pomiferin
Growth inhibition of human HCT15 cells after 21 hrs by MTT assay
Growth inhibition of human HCT15 cells after 21 hrs by MTT assay
[PMID: 17662606]
Hepatocyte GI50
123 μM
Compound: 2, Pomiferin
Growth inhibition of human hepatocytes after 21 hrs by MTT assay
Growth inhibition of human hepatocytes after 21 hrs by MTT assay
[PMID: 17662606]
LOX IMVI GI50
3.34 μM
Compound: 2, Pomiferin
Growth inhibition of human LOX-IMVI cells after 21 hrs by MTT assay
Growth inhibition of human LOX-IMVI cells after 21 hrs by MTT assay
[PMID: 17662606]
MDA-MB-231 GI50
2.92 μM
Compound: 2, Pomiferin
Growth inhibition of human MDA-MB-231 cells after 21 hrs by MTT assay
Growth inhibition of human MDA-MB-231 cells after 21 hrs by MTT assay
[PMID: 17662606]
NCI-H23 GI50
5.14 μM
Compound: 2, Pomiferin
Growth inhibition of human NCI-H23 cells after 21 hrs by MTT assay
Growth inhibition of human NCI-H23 cells after 21 hrs by MTT assay
[PMID: 17662606]
PC-3 GI50
3.95 μM
Compound: 2, Pomiferin
Growth inhibition of human PC3 cells after 21 hrs by MTT assay
Growth inhibition of human PC3 cells after 21 hrs by MTT assay
[PMID: 17662606]
体外研究
(In Vitro)

Pomiferin is an potential inhibitor of HDAC, with an IC50 of 1.05 μM. Pomiferin shows cytotoxic effects on human tumor cell lines, with GI50s of 1.32 ± 0.02 μM (HCT-15 cells), 2.92 ± 0.09 μM (MDA-MB-231 cells), 3.18 ± 0.05 μM (ACHN cells), 3.34 ± 0.11 μM (LOX-IMVI cells), 3.95 ± 0.05 μM (PC-3 cells), 5.14 ± 0.06 μM (NCI-H23 cells), and 123 μM (Hepatocyte cells)[1]. Pomiferin is a highly specific mTOR inhibitor, with an IC50 of 6.2 µM. Pomiferin triacetate only affects two PI3Kα mutants, E542K and E545K. Pomiferin triacetate (0.3125-20 µM) stabilizes Pdcd4 from TPA-induced degradation in HEK293 cells. Pomiferin triacetate (20 µM) inhibits IGF-1-induced signaling downstream of Akt activation[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Pomiferin 相关抗体:
体内研究
(In Vivo)

Pomiferin (5, 10 and 20 mg/kg, p.o.) shows protective effects on the treatment of reperfusion injury. Pomiferin also increases SOD activities and total antioxidative capacity, and decreases malondialdehyde in rats[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

420.45

Formula

C25H24O6
CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

橙桑黄酮
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 8.5 mg/mL (20.22 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3784 mL 11.8920 mL 23.7840 mL
5 mM 0.4757 mL 2.3784 mL 4.7568 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 98.98%

COA (291 KB) HNMR (212 KB) RP-HPLC (261 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[1]

Cell lines purchased from ATCC are maintained in Dulbecco’s modified Eagle’s media (DMEM) supplemented with 10% horse serum and 5% fetal bovine serum and incubated in a CO2 incubator (5%) at 37°C. Cells are serum-deprived by three washes of PBS and resuspended in DMEM. The suspended cells are plated on 96-well plates (1 × 104 cells/well) and treated with Pomiferin. After treatment for 21 h, MTT is added to the medium (0.5 mg/mL), and the mixture is incubated at 37°C for another 3 h. After discarding the medium, DMSO (100 mL) is then applied to the well to dissolve the formazan crystals, and the absorbances at 570 and 630 nm in each well are measured on a micro-ELISA reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3]

Rats[3]
Male Wistar SPF laboratory rats are used in the study. After 10 days of acclimation, 50 animals are randomised in 5 groups (n=10). One group is left intact. Four groups undergo laparotomy in general anaesthesia (2% Rometar (xylazine) 0.5 mL + Narkamon (ketamine) 10 mL, dose 0.5 mL solution /100 g of body mass). Ischaemia-reperfusion injury is induced by applying a vascular clamp on the left renal artery for 60 min with subsequent renal reperfusion. Each animal (including those of the intact group) is put into its own metabolic cage. For 15 days all the animals are bred in these cages. The doses of pomiferin are suspended in 2 mL of 0.5% Avicel solution (microcrystalline cellulose) and are administered starting with day 1 after operation. Three of four operated groups are medicated with Pomiferin-orally by gastric gavage once a day in different doses: 5 mg/kg, 10 mg/kg and 20 mg/kg. The placebo group is given 2 mL of 0.5% Avicel by the same route. On day 15, all the animals are exsanguinated in general anaesthesia (2% Rometar 0.5 mL + Narkamon 10 mL, dose 0.5 mL solution /100 g of the rat body mass) by blood collection from the left ventricle and the reperfused kidney tissue is employed for histopathological examination[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Pomiferin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3784 mL 11.8920 mL 23.7840 mL 59.4601 mL
5 mM 0.4757 mL 2.3784 mL 4.7568 mL 11.8920 mL
10 mM 0.2378 mL 1.1892 mL 2.3784 mL 5.9460 mL
15 mM 0.1586 mL 0.7928 mL 1.5856 mL 3.9640 mL
20 mM 0.1189 mL 0.5946 mL 1.1892 mL 2.9730 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pomiferin572-03-2NSC 5113橙桑黄酮NSC5113NSC-5113HDACmTORHistone deacetylasesMammalian target of RapamycinInhibitorinhibitorinhibit

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