2. Cell Cycle/DNA Damage Anti-infection
  3. DNA/RNA Synthesis Orthopoxvirus
  4. Alovudine

Alovudine  (Synonyms: 3'-Fluoro-3'-deoxythymidine; 3′-Deoxy-3′-fluorothymidine; FLT)

目录号: HY-B1516 纯度: 99.88%
COA 产品使用指南

摩尔计算器

Alovudine (3'-Fluoro-3'-deoxythymidine) 是 DNA 合成的标志物，其比 18F-Fluorodeoxyglucose (FDG) 更不易受炎症变化的影响，因此在胰腺癌中是更好的生物标志物。Alovudine 具有抗正痘活性。

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Alovudine Chemical Structure

Alovudine Chemical Structure

CAS No. : 25526-93-6

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规格 价格 是否有货 数量
Free Sample (0.1 - 0.2 mg)   Apply now  
10 mM * 1 mL in DMSO ￥308
In-stock
5 mg ￥280
In-stock
10 mg ￥415
In-stock
25 mg ￥750
In-stock
50 mg ￥1200
In-stock
100 mg ￥1600
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Alovudine 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Alovudine (3'-Fluoro-3'-deoxythymidine) is a marker of DNA synthesis that is less susceptible to inflammatory changes than 18F-Fluorodeoxyglucose (FDG) and thus is a better biomarker in pancreatic cancer. Alovudine shows anti-orthopoxvirus activity[1][2].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
CCRF-CEM IC50
> 250 μM
Compound: 1, FLT
Cytostatic activity against thymidine kinase-deficient human CEM cells assessed as inhibition of cell proliferation after 48 hrs by Coulter counter analysis
Cytostatic activity against thymidine kinase-deficient human CEM cells assessed as inhibition of cell proliferation after 48 hrs by Coulter counter analysis
[PMID: 24751439]
CCRF-CEM EC50
> 50 μM
Compound: 1, FLT
Antiretroviral activity against HIV-2 ROD infected in thymidine kinase-deficient human CEM cells assessed as inhibition of virus-induced giant cell formation after 4 to 5 days by microscopic analysis
Antiretroviral activity against HIV-2 ROD infected in thymidine kinase-deficient human CEM cells assessed as inhibition of virus-induced giant cell formation after 4 to 5 days by microscopic analysis
[PMID: 24751439]
CCRF-CEM IC50
39 μM
Compound: 1, FLT
Cytostatic activity against human CEM/0 cells expressing thymidine kinase assessed as inhibition of cell proliferation after 48 hrs by Coulter counter analysis
Cytostatic activity against human CEM/0 cells expressing thymidine kinase assessed as inhibition of cell proliferation after 48 hrs by Coulter counter analysis
[PMID: 24751439]
CEM-SS EC50
20 nM
Compound: 1, FLT
Antiviral activity against HIV-1 subtype 3B infected in CEM-SS cells assessed as inhibition of viral replication after 6 days by XTT assay
Antiviral activity against HIV-1 subtype 3B infected in CEM-SS cells assessed as inhibition of viral replication after 6 days by XTT assay
[PMID: 22858097]
HeLa EC50
> 400 μM
Compound: 1, FLT, Alovudine
Cytotoxicity against human HeLa P4/R5 cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa P4/R5 cells after 48 hrs by MTT assay
[PMID: 22352809]
HeLa EC50
0.4 μM
Compound: 1, FLT, Alovudine
Antiviral activity against HIV1 BaL infected in HeLa P4/R5 cells assessed as reduction of viral infection incubated for 2 hrs followed by incubated in drug-free medium for 46 hrs by single round infection beta-galactosidase reporter gene assay
Antiviral activity against HIV1 BaL infected in HeLa P4/R5 cells assessed as reduction of viral infection incubated for 2 hrs followed by incubated in drug-free medium for 46 hrs by single round infection beta-galactosidase reporter gene assay
[PMID: 22352809]
HeLa EC50
0.8 μM
Compound: 1, FLT, Alovudine
Antiviral activity against HIV1 3B infected in HeLa P4/R5 cells assessed as reduction of viral infection incubated for 2 hrs followed by incubated in drug-free medium for 46 hrs by single round infection beta-galactosidase reporter gene assay
Antiviral activity against HIV1 3B infected in HeLa P4/R5 cells assessed as reduction of viral infection incubated for 2 hrs followed by incubated in drug-free medium for 46 hrs by single round infection beta-galactosidase reporter gene assay
[PMID: 22352809]
L1210 IC50
0.081 μM
Compound: 1, FLT
Cytostatic activity against mouse L1210 cells assessed as inhibition of cell proliferation after 48 hrs by Coulter counter analysis
Cytostatic activity against mouse L1210 cells assessed as inhibition of cell proliferation after 48 hrs by Coulter counter analysis
[PMID: 24751439]
MT4 CC50
0.197 μM
Compound: FddThd
Cytotoxic concentration required to reduce the viability of normal uninfected MT-4 cells by 50%
Cytotoxic concentration required to reduce the viability of normal uninfected MT-4 cells by 50%
[PMID: 1697345]
Clinical Trial
分子量

244.22

Formula

C10H13FN2O4
CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (409.47 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : ≥ 25 mg/mL (102.37 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.0947 mL 20.4733 mL 40.9467 mL
5 mM 0.8189 mL 4.0947 mL 8.1893 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.52 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.52 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.88%

COA (289 KB) HNMR (259 KB) LCMS (91 KB)

产品使用指南 (1538 KB)
参考文献

Alovudine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.0947 mL 20.4733 mL 40.9467 mL 102.3667 mL
5 mM 0.8189 mL 4.0947 mL 8.1893 mL 20.4733 mL
10 mM 0.4095 mL 2.0473 mL 4.0947 mL 10.2367 mL
15 mM 0.2730 mL 1.3649 mL 2.7298 mL 6.8244 mL
20 mM 0.2047 mL 1.0237 mL 2.0473 mL 5.1183 mL
25 mM 0.1638 mL 0.8189 mL 1.6379 mL 4.0947 mL
30 mM 0.1365 mL 0.6824 mL 1.3649 mL 3.4122 mL
40 mM 0.1024 mL 0.5118 mL 1.0237 mL 2.5592 mL
50 mM 0.0819 mL 0.4095 mL 0.8189 mL 2.0473 mL
60 mM 0.0682 mL 0.3412 mL 0.6824 mL 1.7061 mL
80 mM 0.0512 mL 0.2559 mL 0.5118 mL 1.2796 mL
100 mM 0.0409 mL 0.2047 mL 0.4095 mL 1.0237 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Alovudine25526-93-63'-Fluoro-3'-deoxythymidine 3′-Deoxy-3′-fluorothymidine FLTDNA/RNA SynthesisOrthopoxvirusInhibitorinhibitorinhibit

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