2. Apoptosis
  3. Apoptosis
  4. BCH

BCH  (Synonyms: 2-Amino-2-norbornanecarboxylic acid; LAT1-IN-1)

目录号: HY-108540 纯度: 99.13%
COA 产品使用指南

摩尔计算器

BCH (2-Amino-2-norbornanecarboxylic acid) 是 L 型氨基酸转运蛋白 1 (LAT1) 的选择性竞争性抑制剂，可显著抑制细胞对氨基酸的摄取和 mTOR 磷酸化，从而抑制癌细胞生长并诱导细胞凋亡。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

BCH Chemical Structure

BCH Chemical Structure

CAS No. : 20448-79-7

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥660
In-stock
50 mg ￥600
In-stock
100 mg ￥1000
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

BCH 相关抗体

Top Publications Citing Use of Products

    BCH purchased from MCE. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    LAT1-IN1 (30 nM) inhibits the expression levels of phosphorylated mTOR and P70S6K in T cells.

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    BCH (2-Amino-2-norbornanecarboxylic acid) is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1) significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis[1][2][3].

    IC50 & Target

    LAT1[1]
    体外研究
    (In Vitro)

    BCH (1-100 mM; 3 days; KYSE30 and KYSE150 esophageal cancer cells) treatment suppresses cell proliferation in a dose-dependent manner[1].
    BCH (30 mM; 24 and 48 hours; KYSE30 and KYSE150 cells) treatment significantly increases cell population in the G0/G1 phase in both KYSE30 and KYSE150 cells, indicating that BCH induces cell cycle arrest at G1 phase[1].
    BCH (30 mM; 0-24 hours; KYSE30 and KYSE150 cells) treatment decreases phosphorylation of 4E-BP1 and p70S6K at 30 minutes and the decrease is continued for 24 hours. The amount of mTOR, 4E-BP1, and p70S6K proteins is slightly decreased[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    BCH 相关抗体:

    Cell Proliferation Assay[1]

    Cell Line: KYSE30 and KYSE150 esophageal cancer cells
    Concentration: 1 mM, 3 mM, 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, or 100 mM
    Incubation Time: 3 days
    Result: Cell proliferation was suppressed in a dose-dependent manner.

    Cell Cycle Analysis[1]

    Cell Line: KYSE30 and KYSE150 cells
    Concentration: 30 mM
    Incubation Time: 24 and 48 hours
    Result: Cell cycle arrest.

    Western Blot Analysis[1]

    Cell Line: KYSE30 and KYSE150 cells
    Concentration: 30 mM
    Incubation Time: 0 hour, 0.5 hour, 1 hour, 3 hours, 6 hours, 24 hours
    Result: Phosphorylation of 4E-BP1 and p70S6K was decreased. The amount of mTOR, 4E-BP1, and p70S6K proteins was slightly decreased.
    体内研究
    (In Vivo)

    BCH (200 mg/kg; intravenous injection; daily; for 14 days; male BALB/c nude mice) treatment significantly delays tumor growth and decreases glucose metabolism, indicating that LAT1 inhibition potentially suppresses esophageal cancer growth in vivo[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male BALB/c nude mice (5-week-old) with KYSE150 cells[1]
    Dosage: 200 mg/kg
    Administration: Intravenous injection; daily; for 14 days
    Result: Significantly delayed tumor growth and decreased glucose metabolism.
    Clinical Trial
    分子量

    155.19

    Formula

    C8H13NO2
    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 20 mg/mL (128.87 mM; 超声助溶 (<60°C))

    DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 6.4437 mL 32.2186 mL 64.4371 mL
    5 mM 1.2887 mL 6.4437 mL 12.8874 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 12.5 mg/mL (80.55 mM); 澄清溶液; 超声助溶 (<60°C)

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.13%

    COA (296 KB) HNMR (246 KB) RP-HPLC (217 KB) MS (83 KB)

    产品使用指南 (1538 KB)
    参考文献

    BCH 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.4437 mL 32.2186 mL 64.4371 mL 161.0929 mL
    5 mM 1.2887 mL 6.4437 mL 12.8874 mL 32.2186 mL
    10 mM 0.6444 mL 3.2219 mL 6.4437 mL 16.1093 mL
    15 mM 0.4296 mL 2.1479 mL 4.2958 mL 10.7395 mL
    20 mM 0.3222 mL 1.6109 mL 3.2219 mL 8.0546 mL
    25 mM 0.2577 mL 1.2887 mL 2.5775 mL 6.4437 mL
    30 mM 0.2148 mL 1.0740 mL 2.1479 mL 5.3698 mL
    40 mM 0.1611 mL 0.8055 mL 1.6109 mL 4.0273 mL
    50 mM 0.1289 mL 0.6444 mL 1.2887 mL 3.2219 mL
    60 mM 0.1074 mL 0.5370 mL 1.0740 mL 2.6849 mL
    80 mM 0.0805 mL 0.4027 mL 0.8055 mL 2.0137 mL
    100 mM 0.0644 mL 0.3222 mL 0.6444 mL 1.6109 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BCH20448-79-72-Amino-2-norbornanecarboxylic acid LAT1-IN-1ApoptosisInhibitorinhibitorinhibit

    您最近查看的产品:

    Your information is safe with us. * Required Fields.

       产品名称：

    		  

    * 需求量：

    * 客户姓名：

    		 

    * Email：

    * 电话：

    		 

    * 公司或机构名称：

       留言给我们：

    Bulk Inquiry

    Inquiry Information

    产品名称：
    BCH
    目录号：
    HY-108540
    需求量：

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

       产品名称：

    		  

    * Lot #：

    * 客户姓名：

    		 

    * Email：

       电话：

    		 

    * 部门：

    * 公司或机构名称：

    		 

       留言给我们：

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.

    嗨！很高兴为您提供帮助！

    尊敬的 MCE 客户您好，
    请您选择所在区域，我们将转接对应客服为您服务！

    热门区域

    A

    B

    C

    F

    G

    H

    J

    L

    N

    Q

    S

    T

    X

    Y

    Z

    热 A B C F G H J L N Q S T X Y Z