2. Apoptosis
  3. Apoptosis
  4. BCH

BCH  (Synonyms: 2-Amino-2-norbornanecarboxylic acid; LAT1-IN-1)

目录号: HY-108540 纯度: 99.13%
COA 产品使用指南 技术支持

BCH (2-Amino-2-norbornanecarboxylic acid) 是 L 型氨基酸转运蛋白 1 (LAT1) 的选择性竞争性抑制剂，可显著抑制细胞对氨基酸的摄取和 mTOR 磷酸化，从而抑制癌细胞生长并诱导细胞凋亡。

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BCH Chemical Structure

BCH Chemical Structure

CAS No. : 20448-79-7

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥660
In-stock
50 mg ￥600
In-stock
100 mg ￥1000
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

BCH 相关抗体

Top Publications Citing Use of Products

    BCH purchased from MCE. Usage Cited in: Front Immunol. 2022 May 19;13:880262.  [Abstract]

    LAT1-IN1 (30 nM) inhibits the expression levels of phosphorylated mTOR and P70S6K in T cells.

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    BCH (2-Amino-2-norbornanecarboxylic acid) is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1) significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis[1][2][3].

    IC50 & Target

    LAT1[1]
    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    LNCaP IC50
    4060 μM
    Compound: BCH
    Inhibition of LAT in human LNCAP cells assessed as inhibition of [3H]-L-leucine transport by liquid scintillation counting analysis
    Inhibition of LAT in human LNCAP cells assessed as inhibition of [3H]-L-leucine transport by liquid scintillation counting analysis
    		[PMID: 25984885]
    MCF7 IC50
    112 μM
    Compound: BCH
    Competitive inhibition of [14C]-L-leucine uptake at LAT1 in human MCF7 cells preincubated for 5 mins followed by addition of substrate measured during 5 mins by liquid scintillation counting
    Competitive inhibition of [14C]-L-leucine uptake at LAT1 in human MCF7 cells preincubated for 5 mins followed by addition of substrate measured during 5 mins by liquid scintillation counting
    		[PMID: 27253989]
    MCF7 IC50
    362 μM
    Compound: BCH
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by resazurin dye-based fluorimetric analysis
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by resazurin dye-based fluorimetric analysis
    		[PMID: 27253989]
    体外研究
    (In Vitro)

    BCH（1-100 mM；3 天）处理以剂量依赖性方式抑制 KYSE30 和 KYSE150 食管癌细胞增殖[1]。
    BCH（30 mM；24 和 48 小时）处理显着增加了 KYSE30 和 KYSE150 细胞中 G0/G1 期的细胞数量，表明 BCH 诱导细胞周期停滞在 G1 期[1]。
    BCH（30 mM；0-24 小时）在 KYSE30 和 KYSE150 细胞中处理 30 分钟时降低 4E-BP1 和 p70S6K 的磷酸化，并且这种降低持续 24 小时。 mTOR、4E-BP1 和 p70S6K 蛋白的数量略有减少[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    BCH 相关抗体:

    Cell Proliferation Assay[1]

    Cell Line: KYSE30 and KYSE150 esophageal cancer cells
    Concentration: 1 mM, 3 mM, 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, or 100 mM
    Incubation Time: 3 days
    Result: Cell proliferation was suppressed in a dose-dependent manner.

    Cell Cycle Analysis[1]

    Cell Line: KYSE30 and KYSE150 cells
    Concentration: 30 mM
    Incubation Time: 24 and 48 hours
    Result: Cell cycle arrest.

    Western Blot Analysis[1]

    Cell Line: KYSE30 and KYSE150 cells
    Concentration: 30 mM
    Incubation Time: 0 hour, 0.5 hour, 1 hour, 3 hours, 6 hours, 24 hours
    Result: Phosphorylation of 4E-BP1 and p70S6K was decreased. The amount of mTOR, 4E-BP1, and p70S6K proteins was slightly decreased.
    体内研究
    (In Vivo)

    BCH（200 mg/kg；静脉注射；每日；连续 14 天；雄性 BALB/c 裸鼠）处理可显著延缓肿瘤生长并降低葡萄糖代谢，表明 LAT1 抑制可能抑制体内食管癌生长[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male BALB/c nude mice (5-week-old) with KYSE150 cells[1]
    Dosage: 200 mg/kg
    Administration: Intravenous injection; daily; for 14 days
    Result: Significantly delayed tumor growth and decreased glucose metabolism.
    Clinical Trial
    分子量

    155.20

    Formula

    C8H13NO2
    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 20 mg/mL (128.87 mM; 超声助溶 (<60°C))

    DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 6.4434 mL 32.2171 mL 64.4342 mL
    5 mM 1.2887 mL 6.4434 mL 12.8868 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 12.5 mg/mL (80.54 mM); 澄清溶液; 超声助溶 (<60°C)

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.13%

    COA (296 KB) HNMR (246 KB) RP-HPLC (217 KB) MS (83 KB)

    产品使用指南 (1538 KB)
    参考文献

    BCH 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.4434 mL 32.2171 mL 64.4342 mL 161.0856 mL
    5 mM 1.2887 mL 6.4434 mL 12.8868 mL 32.2171 mL
    10 mM 0.6443 mL 3.2217 mL 6.4434 mL 16.1086 mL
    15 mM 0.4296 mL 2.1478 mL 4.2956 mL 10.7390 mL
    20 mM 0.3222 mL 1.6109 mL 3.2217 mL 8.0543 mL
    25 mM 0.2577 mL 1.2887 mL 2.5774 mL 6.4434 mL
    30 mM 0.2148 mL 1.0739 mL 2.1478 mL 5.3695 mL
    40 mM 0.1611 mL 0.8054 mL 1.6109 mL 4.0271 mL
    50 mM 0.1289 mL 0.6443 mL 1.2887 mL 3.2217 mL
    60 mM 0.1074 mL 0.5370 mL 1.0739 mL 2.6848 mL
    80 mM 0.0805 mL 0.4027 mL 0.8054 mL 2.0136 mL
    100 mM 0.0644 mL 0.3222 mL 0.6443 mL 1.6109 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BCH20448-79-72-Amino-2-norbornanecarboxylic acid LAT1-IN-1ApoptosisInhibitorinhibitorinhibit

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