1. Biology Dictionary
  2. Affinity Chromatography Technology

Affinity Chromatography Technology亲和色谱技术

释义:

This class of approaches is characterised by the use of affinity resins as tools to purify molecule of interest (baits) and their binding partners. The baits can be captured by a variety of high affinity ligands linked to a resin - for example, antibodies specific for the bait itself, antibodies for specific tags engineered to be expressed as part of the bait or other high affinity binders such as glutathione resins for GST fusion proteins, metal resins for histidine-tagged proteins.

这一类方法的特点是使用亲和树脂作为工具来纯化感兴趣的分子 (诱饵) 及其结合伙伴。诱饵可以通过与树脂连接的各种高亲和力配体被捕获——例如,针对诱饵本身的特异性抗体、针对诱饵部分表达的特定标签的抗体,或其他高亲和力结合物,如用于 GST 融合蛋白的谷胱甘肽树脂,或用于组氨酸标签蛋白的金属树脂。

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