1. Biology Dictionary
  2. Bimolecular Fluorescence Complementation

Bimolecular Fluorescence Complementation双分子荧光互补法

释义:

The bimolecular fluorescence complementation (BiFC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two non- fluorescent fragments of a protein fluorophore such as green fluorescent protein (GFP) or its derivatives. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional flourophore that can be identified through fluorescence spectroscopy or microscopy.

双分子荧光互补 (BiFC) 是一种用于确定蛋白质相互作用和/或其在活细胞中定位的测定法。该方法基于两个非荧光蛋白质荧光分子片段 (如绿色荧光蛋白 (GFP) 或其衍生物) 之间的互补。融合到每个片段的蛋白质之间的相互作用使片段靠得足够近,从而重新组装并成为功能性的荧光分子,可以通过荧光光谱或显微镜进行鉴定。

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