1. Biology Dictionary
  2. Coimmunoprecipitation

Coimmunoprecipitation免疫共沉淀

释义:

In this approach an antibody, specific for the molecule of interest (bait) or any tag expressed within a fusion protein, is used to separate the bait from a molecular mixture or a cell lysate and to capture its ligand simultaneously. The partners that bind to the bait molecule retained by the resin can then be eluted and identified. The antibody may be free or bound to a matrix during this process.

在这种方法中,特异性抗体用于分离感兴趣的分子 (诱饵) 或任何在融合蛋白中表达的标签,并同时捕获其配体。与树脂结合的诱饵分子所结合的伙伴可以被洗脱并鉴定。抗体可以是自由的,也可以在此过程中与基质结合。

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