1. Biology Dictionary
  2. Electrophoretic Mobility Shift Assay

Electrophoretic Mobility Shift Assay电泳迁移率变动实验

释义:

This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). The nucleic acid is often radio-labelled to enable visualisation by autoradiography. Comparison of the nucleic acid migration in the two lanes enables the retardation of the nucleic acid due to its interaction with a protein to be observed.

该方法证明了核酸与蛋白质伙伴之间的相互作用。在同一凝胶电泳中,一条通道加载已知序列的核酸,另一条通道加载相同的核酸与纯化的蛋白质 (或蛋白质混合物)。 核酸通常会进行放射性标记,以便通过自动放射成像进行可视化。比较两条通道中核酸的迁移可以观察到核酸由于与蛋白质的相互作用而减速的情况。

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