2. Biology Dictionary
  3. Filter Overlay Assay

Filter Overlay Assay滤膜结合法

释义：

A method in which separation depends upon the ability of one participant to bind to a filter or membrane which the other participants do not. Molecules interacting with the bound molecule will also be retain on the filter. For example, proteins expressed by different clones of an expression library are bound to a nitrocellulose membrane, by colony (bacterial library) or plaque (phage library) blotting. A labelled protein can then be used as a probe to identify clones expressing proteins that interact with the probe. Interactions occur on the nitrocellulose filters. The method is highly general and therefore widely applicable. A variety of approaches can be used to label the ligand, alternatively the ligand can be detected by a specific antibody.

该方法依赖于一个参与者能绑定到过滤器或膜上的能力，而其他参与者不能绑定。与绑定分子相互作用的分子也会被保留在过滤器上。例如，通过细菌或噬菌体文库 (colony 或 plaque blotting)， 不同克隆表达的蛋白质被固定在硝酸纤维素膜上。然后使用标记的蛋白质作为探针，识别与探针相互作用的克隆。此方法具有广泛的通用性，适用于多种方法标记配体，或通过特定抗体检测配体。

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