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  2. Mammalian Protein-Protein Interaction Trap

Mammalian Protein-Protein Interaction Trap哺乳动物蛋白互作捕获法

释义:

Mammalian Protein-Protein Interaction Trap (MAPPIT) is a protein-protein interaction screening method based on the reconstruction of membrane STAT (transcriptional activator) receptors in mammalian cells. The bait protein is fused with a receptor lacking the STAT recruitment site, while the prey protein is fused with a functional STAT recruitment site. In this configuration, the given bait-prey interaction restores a STAT-dependent response, leading to the expression of a reporter gene. This system can not only display protein interactions but also interactions that are independent of modifications but depend on tyrosine phosphorylation.

MAPPIT (哺乳动物蛋白质-蛋白质相互作用捕获法) 是一种基于哺乳动物细胞中的膜 STAT (转录激活因子) 受体重建的蛋白质-蛋白质相互作用筛选方法。诱饵蛋白与缺乏 STAT 招募位点的受体融合,而猎物蛋白与功能性 STAT 招募位点融合。在这种配置中,给定的诱饵-猎物相互作用恢复了依赖 STAT 的反应,导致报告基因的表达。该系统不仅能展示蛋白质相互作用,还能展示不依赖修饰且依赖酪氨酸磷酸化的相互作用。

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