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  3. Protein Three Hybrid

Protein Three Hybrid蛋白质三重杂交法

释义：

Protein Three Hybrid is based on the classical two-hybrid technique with the addition of a third protein component to detect interactions among the three. In the experiment, the bait protein, prey protein, and the third protein are co-transfected into recombinant yeast. Negative control experiments show that the interaction between bait and prey does not occur unless the third protein is co-transfected. This technique is commonly used to study the assembly and regulatory mechanisms of multi-protein complexes.

蛋白质三重杂交法通过在经典双杂交技术基础上加入第三种蛋白组分，用于检测三者之间的相互作用。实验将诱饵蛋白、猎物蛋白及第三种蛋白共转染至重组酵母中。阴性对照实验显示，当未转染第三种蛋白时，诱饵与猎物间的相互作用不会发生。此技术常用于研究多蛋白复合物的组装及调控机制。

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