1. Biology Dictionary
  2. Reverse Phase Chromatography

Reverse Phase Chromatography反相色谱法

释义:

Reverse Phase Chromatography is used to separate mixtures of molecules with different hydrophobic and hydrophilic properties. The technique uses silica gel matrices with covalently bound n-alkyl chains as the stationary phase. For example, C-18 columns strongly retain hydrophobic compounds, while hydrophilic molecules elute more quickly. This method is widely used in the separation and purification of proteins, peptides, and small molecule drugs.

反相色谱法可用于分离具有不同疏水性和亲水性的分子混合物。该技术利用带有共价结合 n-烷基链的硅胶基质作为固定相。例如,C-18 色谱柱可强烈保留疏水性化合物,亲水性分子则更快速洗脱。该方法广泛用于蛋白质、多肽和小分子药物的分离纯化。

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