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Solution State Nuclear Magnetic Resonance溶液态核磁共振

释义:

Solution State Nuclear Magnetic Resonance is used to analyze the types, quantities, and arrangements of atoms in chemical systems, liquids, and solids. It is widely applied in molecular structure analysis and dynamics studies. Solution-state NMR requires the sample to be dissolved in deuterated solvents, averaging anisotropic interactions through rapid random rotation, generating high-resolution spectra. However, for macromolecules over 30 kDa, signal complexity and solubility limitations make complete structural determination difficult.

溶液态核磁共振可用于分析化学系统、液体和固体中原子的类型、数量和排列,广泛应用于分子结构解析和动力学研究。溶液态 NMR 要求样品溶解在重氢化溶剂中,通过快速随机旋转平均化各向异性相互作用,生成高分辨率光谱。但对于超过 30 kDa 的大分子,信号复杂性和溶解度限制使得完整结构测定变得困难。

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