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  3. Three Hybrid Assay

Three Hybrid Assay三杂交测定

释义：

The Three Hybrid Assay is used to study the role of third-party molecules in the binding process between bait and prey, particularly significant in transcriptional regulation and protein complex assembly research. This method is based on a complementation experiment, indicating that a third-party molecule is necessary for the binding of a specific bait-prey pair. The molecule fused with the DNA binding domain is the bait, while the molecule fused with the transcriptional activation factor is the prey, revealing complex molecular interaction processes.

三杂交测定可用于研究诱饵-猎物结合过程中第三方分子的作用机制，尤其在转录调控和蛋白复合物组装研究中具有重要意义。该方法基于互补实验，表明某一第三方分子对特定诱饵-猎物配对的结合是必需的。与 DNA 结合域融合的分子是诱饵，而与转录激活因子融合的分子是猎物，通过这种方式揭示复杂的分子交互过程。

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