2. Academic Validation
  3. Synthesis of fluorophore and quencher monomers for use in scorpion primers and nucleic acid structural probes

Synthesis of fluorophore and quencher monomers for use in scorpion primers and nucleic acid structural probes

  • Org Biomol Chem. 2003 Jul 7;1(13):2267-75. doi: 10.1039/b301859h.
Catherine M McKeen 1,  Lynda J Brown,  Jamie T G Nicol,  John M Mellor,  Tom Brown
Affiliations

Affiliation

  • 1 Department of Chemistry, University of Southampton, Highfield, Southampton, UK S017 1BJ.
PMID: 12945696 DOI: 10.1039/b301859h
Abstract

We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified Oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic Oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.

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