2. Academic Validation
  3. Endonucleases

Endonucleases

  • Curr Protoc Mol Biol. 2011 Jan:Chapter 3:Unit3.12. doi: 10.1002/0471142727.mb0312s93.
Nicole M Nichols 1
Affiliations

Affiliation

  • 1 New England Biolabs, Inc., Ipswich, MA, USA.
PMID: 21225639 DOI: 10.1002/0471142727.mb0312s93
Abstract

Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also provided. For a detailed discussion of the endonucleases more typically associated with DNA damage repair (e.g., Endo III, IV, V and VIII of E. coli and human APE1), see UNIT 3.9.

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