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  3. Interleukin-11 alters placentation and causes preeclampsia features in mice

Interleukin-11 alters placentation and causes preeclampsia features in mice

  • Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):15928-33. doi: 10.1073/pnas.1515076112.
Amy L Winship 1,  Kaori Koga 2,  Ellen Menkhorst 3,  Michelle Van Sinderen 3,  Katarzyna Rainczuk 3,  Miwako Nagai 2,  Carly Cuman 3,  Joanne Yap 4,  Jian-Guo Zhang 5,  David Simmons 6,  Morag J Young 7,  Evdokia Dimitriadis 8
Affiliations

Affiliations

  • 1 Embryo Implantation Laboratory, Hudson Institute, Clayton, VIC 3168, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC 3800, Australia; Department of Molecular and Translational Medicine, Monash University, Clayton, VIC 3800, Australia;
  • 2 Obstetrics and Gynecology, University of Tokyo, Tokyo 113-8655, Japan;
  • 3 Embryo Implantation Laboratory, Hudson Institute, Clayton, VIC 3168, Australia; Department of Molecular and Translational Medicine, Monash University, Clayton, VIC 3800, Australia;
  • 4 Embryo Implantation Laboratory, Hudson Institute, Clayton, VIC 3168, Australia;
  • 5 Cancer and Haemotology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia;
  • 6 School of Biomedical Sciences, University of Queensland, QLD 4072, Australia;
  • 7 Department of Molecular and Translational Medicine, Monash University, Clayton, VIC 3800, Australia; Cardiovascular Endocrinology Lab, Hudson Institute, Clayton, VIC 3168, Australia.
  • 8 Embryo Implantation Laboratory, Hudson Institute, Clayton, VIC 3168, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC 3800, Australia; Department of Molecular and Translational Medicine, Monash University, Clayton, VIC 3800, Australia; evdokia.dimitriadis@hudson.org.au.
PMID: 26655736 DOI: 10.1073/pnas.1515076112
Abstract

Preeclampsia (PE) is a pregnancy-specific disorder characterized by hypertension and proteinuria after 20 wk gestation. Abnormal extravillous trophoblast (EVT) invasion and remodeling of uterine spiral arterioles is thought to contribute to PE development. Interleukin-11 (IL11) impedes human EVT invasion in vitro and is elevated in PE decidua in women. We demonstrate that IL11 administered to mice causes development of PE features. Immunohistochemistry shows IL11 compromises trophoblast invasion, spiral artery remodeling, and placentation, leading to increased systolic blood pressure (SBP), proteinuria, and intrauterine growth restriction, although nonpregnant mice were unaffected. Real-Time PCR array analysis identified pregnancy-associated plasma protein A2 (PAPPA2), associated with PE in women, as an IL11 regulated target. IL11 increased PAPPA2 serum and placental tissue levels in mice. In vitro, IL11 compromised primary human EVT invasion, whereas siRNA knockdown of PAPPA2 alleviated the effect. Genes regulating uterine natural killer (uNK) recruitment and differentiation were down-regulated and uNK cells were reduced after IL11 treatment in mice. IL11 withdrawal in mice at onset of PE features reduced SBP and proteinuria to control levels and alleviated placental labyrinth defects. In women, placental IL11 immunostaining levels increased in PE pregnancies and in serum collected from women before development of early-onset PE, shown by ELISA. These results indicate that elevated IL11 levels result in physiological changes at the maternal-fetal interface, contribute to abnormal placentation, and lead to the development of PE. Targeting placental IL11 may provide a new treatment option for PE.

Keywords

cytokines; placenta; pregnancy; trophoblast.

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