2. Academic Validation
  3. Liquid Chromatography-Mass Spectrometry-Based Rapid Secondary-Metabolite Profiling of Marine Pseudoalteromonas sp. M2

Liquid Chromatography-Mass Spectrometry-Based Rapid Secondary-Metabolite Profiling of Marine Pseudoalteromonas sp. M2

  • Mar Drugs. 2016 Jan 20;14(1):24. doi: 10.3390/md14010024.
Woo Jung Kim 1,  Young Ok Kim 2,  Jin Hee Kim 3,  Bo-Hye Nam 4,  Dong-Gyun Kim 5,  Cheul Min An 6,  Jun Sik Lee 7,  Pan Soo Kim 8,  Hye Min Lee 9,  Joa-Sup Oh 10,  Jong Suk Lee 11
Affiliations

Affiliations

  • 1 Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon, Gyeonggi-do 16229, Korea. wj0504@gstep.re.kr.
  • 2 Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang-gun, Busan 46083, Korea. yobest12@korea.kr.
  • 3 College of Herbal Bio-Industry, Daegu Haany University, Gyeongsan, Gyeongbuk 42158, Korea. jinheekim@dhu.ac.kr.
  • 4 Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang-gun, Busan 46083, Korea. nambohye@korea.kr.
  • 5 Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang-gun, Busan 46083, Korea. combikola@korea.kr.
  • 6 Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang-gun, Busan 46083, Korea. ancm@korea.kr.
  • 7 Department of Biology, Immunology Research Lab, BK21-plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Dong-gu, Gwangju 61452, Korea. junsiklee@chosun.ac.kr.
  • 8 Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon, Gyeonggi-do 16229, Korea. pan@gstep.re.kr.
  • 9 Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon, Gyeonggi-do 16229, Korea. hmlee@gstep.re.kr.
  • 10 Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon, Gyeonggi-do 16229, Korea. jsoh@gstep.re.kr.
  • 11 Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon, Gyeonggi-do 16229, Korea. jslee@gstep.re.kr.
PMID: 26805856 DOI: 10.3390/md14010024
Abstract

The ocean is a rich resource of flora, fauna, and food. A wild-type Bacterial strain showing confluent growth on marine agar with Antibacterial activity was isolated from marine water, identified using 16S rDNA sequence analysis as Pseudoalteromonas sp., and designated as strain M2. This strain was found to produce various secondary metabolites including quinolone Alkaloids. Using high-resolution mass spectrometry (MS) and nuclear magnetic resonance (NMR) analysis, we identified nine secondary metabolites of 4-hydroxy-2-alkylquinoline (pseudane-III, IV, V, VI, VII, VIII, IX, X, and XI). Additionally, this strain produced two novel, closely related compounds, 2-isopentylqunoline-4-one and 2-(2,3-dimetylbutyl)qunoline-4-(1H)-one, which have not been previously reported from marine bacteria. From the metabolites produced by Pseudoalteromonas sp. M2, 2-(2,3-dimethylbutyl)quinolin-4-one, pseudane-VI, and pseudane-VII inhibited melanin synthesis in Melan-A cells by 23.0%, 28.2%, and 42.7%, respectively, wherein pseudane-VII showed the highest inhibition at 8 µg/mL. The results of this study suggest that liquid chromatography (LC)-MS/MS-based metabolite screening effectively improves the efficiency of novel metabolite discovery. Additionally, these compounds are promising candidates for further bioactivity development.

Keywords

Pseudoalteromonas; marine microbes; mass spectrometry; quinolone alkaloid; secondary metabolite.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z