2. Academic Validation
  3. Development of a chemical fingerprint as a tool to distinguish closely related Tinospora species and quantitation of marker compounds

Development of a chemical fingerprint as a tool to distinguish closely related Tinospora species and quantitation of marker compounds

  • J Pharm Biomed Anal. 2020 Jan 30:178:112894. doi: 10.1016/j.jpba.2019.112894.
Abidah Parveen 1,  Yan-Hong Wang 2,  Omer Fantoukh 3,  Manal Alhusban 4,  Vijayasankar Raman 2,  Zulfiqar Ali 2,  Ikhlas A Khan 5
Affiliations

Affiliations

  • 1 Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; Department of Pharmacy, Abbottabad University of Science & Technology, Havelian, KPK, Pakistan.
  • 2 National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA.
  • 3 Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, 4545, Saudi Arabia.
  • 4 Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA.
  • 5 Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA. Electronic address: ikhan@olemiss.edu.
PMID: 31606561 DOI: 10.1016/j.jpba.2019.112894
Abstract

Tinospora species are morphologically similar. Several cases of human toxicity have been reported in association with T. crispa. A chemical fingerprint was developed to differentiate T. crispa from its closely related species and to quantitate its major furanoditerpenes namely as borapetosides B, C and F. The rapid, sensitive and repeatable method was established using ultra-high performance liquid chromatography coupled with photodiode array and single quadrupole electrospray mass spectrometry detectors using a flavonoid, two Alkaloids, an amide and six Diterpenoids. Qualitative and quantitative determination was performed by UHPLC-UV and confirmed by MS. The intra-day RSD for replicates was between 0.9 and 6.8% and inter-day RSD was between 1.2 and 9.1%. Recovery was 97-103 %. The method is useful to achieve decisiveness in not only identifying but also differentiating T. crispa from T. sinensis and other closely related Tinospora species. Seventeen Tinospora plant samples and seventeen dietary supplements claiming T. crispa, T. sinensis and T. cordifolia were analyzed. The newly developed and validated method successfully resulted in the conclusive identification of two dietary supplements to be mislabeled.

Keywords

Borapetoside C; Dietary supplements; Magnoflorine; T. crispa; T. sinensis; Tinosineside A.

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