2. Academic Validation
  3. Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays

Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays

  • STAR Protoc. 2023 Jul 31;4(3):102477. doi: 10.1016/j.xpro.2023.102477.
Samantha Y Tse 1,  Read Pukkila-Worley 2
Affiliations

Affiliations

  • 1 Program in Innate Immunity, Division of Infectious Diseases and Immunology, Department of Medicine, UMass Chan Medical School, Worcester, MA 01655, USA.
  • 2 Program in Innate Immunity, Division of Infectious Diseases and Immunology, Department of Medicine, UMass Chan Medical School, Worcester, MA 01655, USA. Electronic address: read.pukkila-worley@umassmed.edu.
PMID: 37527042 DOI: 10.1016/j.xpro.2023.102477
Abstract

The Caenorhabditis elegans genome encodes a greatly expanded number of nuclear hormone receptors, many of which remain orphaned. Here, we present a protocol to assess ligand-receptor binding in C. elegans using an adapted cellular thermal shift assay and isothermal dose response. We describe steps for growing C. elegans and preparing lysates and compounds. We also detail how to perform and quantify these assays. This protocol can be used to study any soluble receptor. For complete details on the use and execution of this protocol, please refer to Peterson et al. (2023).1.

Keywords

Model Organisms; Molecular Biology; Protein Biochemistry.

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