2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. OTUB2-IN-1

OTUB2-IN-1 是 OTUB2 的特异性抑制剂 (KD 约为12 μM)， 可以通过促进 细胞毒性 T 淋巴细胞 (CTL) 的强大肿瘤内浸润来降低肿瘤细胞中 PD-L1 蛋白的表达并抑制肿瘤生长。

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OTUB2-IN-1 Chemical Structure

OTUB2-IN-1 Chemical Structure

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规格 价格 是否有货 数量
5 mg ￥880
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10 mg ￥1400
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25 mg ￥2800
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50 mg ￥4480
In-stock
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Customer Review

OTUB2-IN-1 相关抗体

Top Publications Citing Use of Products

查看 Deubiquitinase 亚型特异性产品:

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USP1 USP2 USP7 USP8 USP30 UCHL1

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

OTUB2-IN-1, a specific inhibitor of OTUB2 (KD: ~12 μM), reduces PD-L1 protein expression in tumor cells and inhibits tumor growth by promoting robust intra-tumor infiltration of cytotoxic T lymphocytes (CTL) [1].

体外研究
(In Vitro)

OTUB2-IN-1 (0-40 μM; 2 h) 能够以剂量依赖性方式降低肿瘤细胞 (NCI-H358，SK-MES-1和 NCI-H226) 中的 PD-L1 水平，但是它不能影响 OTUB2 的稳定性[1]。
OTUB2-IN-1 (0-50 μM; 1 h) 不干扰 OTUB2 和 PD-L1 之间的蛋白质相互作用[1]。
OTUB2-IN-1 (10 μM; 0-4 d) 对 B16-F10 肿瘤细胞的活力没有明显的抑制作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

OTUB2-IN-1 相关抗体:

Cell Viability Assay[1]

Cell Line: B16-F10 tumor cells
Concentration: 10 μM
Incubation Time: 0-4 d
Result: OTUB2-IN-1 did not show significant inhibitory effects on the viability of B16-F10 tumor cells over a period of up to four days.

Western Blot Analysis[1]

Cell Line: NCI-H358，SK-MES-1, NCI-H226
Concentration: 0-40 μM
Incubation Time:
Result: Reduced PD-L1 levels in a dose-dependent manner across these cell lines, indicating effective inhibition of OTUB2's functional activity regarding PD-L1 stabilization.
However, it did not affect the stability of OTUB2 itself, suggesting that the inhibitor specifically disrupts the PD-L1 regulatory function of OTUB2 without altering OTUB2 protein stability.
体内研究
(In Vivo)

OTUB2-IN-1 (20 mg/kg; i.p.; 每天一次持续五天), 在植入 B16-F10细胞的小鼠中，提示其具有增强肿瘤细胞免疫识别和免疫应答的潜能[1]。
OTUB2-IN-1 （20 mg/kg; i.p.; 每天一次持续五天） 在植入 LL/2 细胞的小鼠中,降低 YAP 和磷酸化的 p65 的表达; 在植入 B16-F10 细胞的小鼠中降低了磷酸化 Akt 的表达； 在植入 KLN205 细胞的小鼠中中降低了磷酸化 p65 的表达[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice implanted with B16-F10 cells or LL/2 cells [1]
Dosage: 20 mg/kg; daily for five days
Administration: i.p
Result: Reduced the expression of PD-L1 on tumor cells, suggesting its potential in enhancing immune recognition and response against tumor cells.
Did not significantly impact tumor cell viability directly, indicating its action might be more about modulating immune evasion mechanisms rather than cytotoxic effects.
Increased the infiltration of cytotoxic T cells within the tumors, suggesting that reducing PD-L1 levels can indeed make the tumor more susceptible to immune attack.within the tumors, suggesting that reducing PD-L1 levels can indeed make the tumor more susceptible to immune attack.
分子量

434.49

Formula

C19H18N2O6S2
性状

固体

颜色

Orange to red

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 50 mg/mL (115.08 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3015 mL 11.5077 mL 23.0155 mL
5 mM 0.4603 mL 2.3015 mL 4.6031 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

OTUB2-IN-1 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3015 mL 11.5077 mL 23.0155 mL 57.5387 mL
5 mM 0.4603 mL 2.3015 mL 4.6031 mL 11.5077 mL
10 mM 0.2302 mL 1.1508 mL 2.3015 mL 5.7539 mL
15 mM 0.1534 mL 0.7672 mL 1.5344 mL 3.8359 mL
20 mM 0.1151 mL 0.5754 mL 1.1508 mL 2.8769 mL
25 mM 0.0921 mL 0.4603 mL 0.9206 mL 2.3015 mL
30 mM 0.0767 mL 0.3836 mL 0.7672 mL 1.9180 mL
40 mM 0.0575 mL 0.2877 mL 0.5754 mL 1.4385 mL
50 mM 0.0460 mL 0.2302 mL 0.4603 mL 1.1508 mL
60 mM 0.0384 mL 0.1918 mL 0.3836 mL 0.9590 mL
80 mM 0.0288 mL 0.1438 mL 0.2877 mL 0.7192 mL
100 mM 0.0230 mL 0.1151 mL 0.2302 mL 0.5754 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OTUB2-IN-1DeubiquitinaseDUBsPD-L1CTLtumors C57BL/6 miceInhibitorinhibitorinhibit

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目录号：
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