1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Pepsin (MS Grade)

Pepsin (MS Grade)  (Synonyms: 胃蛋白酶(MS 级); EC 3.4.23.1 (MS Grade))

目录号: HY-E70198
COA 产品使用指南

Pepsin (MS Grade) 是人和猪胃中的主要蛋白酶,是一种类似胃蛋白酶的胃小蛋白水解酶。Pepsin (MS Grade) 有助于脊椎动物胃中食物蛋白质的蛋白质水解。

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Pepsin (MS Grade) Chemical Structure

Pepsin (MS Grade) Chemical Structure

CAS No. : 9001-75-6

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250 mg ¥2800
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Other Forms of Pepsin (MS Grade):

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Pepsin (MS Grade) is the major pig and human gastric proteases, it is a pepsin-like minor gastric proteolytic enzymes. Pepsin (MS Grade) contributes to proteolysis of food proteins in the vertebrate stomach[1].

体外研究
(In Vitro)

本产品可用于:1) 质谱分析和其他应用中进行蛋白分析;2)用于结构蛋白研究(HDX 交换)和抗体分析;3)用于消化蛋白质组学上紧密折叠蛋白;4)疫苗生产消化酪蛋白。
使用方法
1)目的蛋白溶解:将蛋白溶解于 pH 1.0-5.0 缓冲液中;
2)酶溶解:将胃蛋白酶粉末溶解于双蒸水中,注意 pH 应低于 5.5,使胃蛋白酶的终浓度为 1 mg/mL;
3)酶切:将胃蛋白酶加入目的蛋白溶液中,推荐比例为酶:蛋白=1:20-1:100(W:W),混匀后 37℃ 反应 1-18 h,95℃ 加热 10 min 终止反应。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号
性状

固体

颜色

Off-white to light yellow

中文名称

胃蛋白酶(MS 级)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pepsin (MS Grade)
目录号:
HY-E70198
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