冰冻切片技术是一种用于在细胞和组织研究中制备切片的方法。首先，组织样本通过冰冻被包裹在冰冻剂中，形成冰冻块。然后，使用冰冻切片机或微tome在极低温下切割薄片。这种技术保留了细胞和组织的天然结构和生物活性，适用于免疫组织化学、荧光显微镜、原位杂交等研究。冰冻切片技术对于保存生物分子的活性和提供高分辨率图像具有重要意义。

Frozen sectioning is a method used to prepare sections for the study of cells and tissues. First, the tissue sample is encapsulated in cryogen by freezing to form a frozen block. Then, use a freezing microtome or microtome to cut thin sections at very low temperatures. This technology retains the natural structure and biological activity of cells and tissues, and is suitable for studies such as immunohistochemistry, fluorescence microscopy, and in situ hybridization. Frozen sectioning technology is important for preserving the activity of biomolecules and providing high-resolution images.