Protopine hydrochloride  (Synonyms: Corydinine hydrochloride)

Protopine hydrochloride, an isoquinoline alkaloid, is a specific reversible and competitive inhibitor of acetylcholinesterase. Protopine hydrochloride exhibits anti-inflammation, anti-microbial, anti-angiogenic and anti-tumour activity^[1][2].

Protopine (10-40 μM，24 h-96 h) hydrochloride 抑制肝癌细胞 (HepG2、Huh7) 的细胞活力、迁移、侵袭和 EMT 过程^[2]。
Protopine (10-40 μM，24 h) hydrochloride 通过增加 HepG2 和 Huh7 细胞中 caspase-3 和 caspase-9 的表达来诱导细胞凋亡，并抑制 PI3K/Akt 信号通路^[2]。
Protopine (10-40 μM，6 小时) hydrochloride 可诱导 HepG2 和 Huh7 细胞中 ROS 的产生^[2]。
Protopine (0-10 μg/mL) hydrochloride 抑制 S6 细胞中血清素转运蛋白 (SERT) 的摄取以及 N1 细胞中去甲肾上腺素 (NE) 的摄取^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Protopine (0.1 和 1 mg/kg，腹腔注射) hydrochloride 可减轻 Scopolamine (HY-N0296) (1 mg/kg) 引起的小鼠记忆障碍^[1]。
Protopine (5-20 mg/kg，静脉注射) hydrochloride 在异种移植 BALB/c 小鼠 (皮下注射 HepG2 或 Huh-7 细胞)中可抑制肿瘤生长并抑制 PI3K/Akt，并诱导 caspase-3 裂解^[2].
Protopine (5-20 mg/kg，腹腔注射) hydrochloride 在小鼠 HTR 和 TST 测试中显示出抗抑郁样作用^[3]。
Protopine (1-4 mg/kg，腹膜内注射，每日一次，持续 3 天) hydrochloride 对大鼠局灶性脑缺血损伤具有保护作用^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

389.83

C₂₀H₂₀ClNO₅

6164-47-2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kim SR, et, al. Protopine from Corydalis ternata has anticholinesterase and antiamnesic activities. Planta Med. 1999 Apr;65(3):218-21.  [Content Brief]

  [2]. Nie C, et, al. Protopine triggers apoptosis via the intrinsic pathway and regulation of ROS/PI3K/Akt signalling pathway in liver carcinoma. Cancer Cell Int. 2021 Jul 27;21(1):396.  [Content Brief]

  [3]. Xu LF, et al. Protopine inhibits serotonin transporter and noradrenaline transporter and has the antidepressant-like effect in mice models. Neuropharmacology. 2006 Jun;50(8):934-40.  [Content Brief]

  [4]. Xiao X, et al. Protective effect of protopine on the focal cerebral ischaemic injury in rats. Basic Clin Pharmacol Toxicol. 2007 Aug;101(2):85-9.  [Content Brief]