FGF-23 蛋白, Rat (His)

FGF-23 protein is a key regulator of phosphate homeostasis and inhibits renal tubular phosphate transport by reducing SLC34A1 levels. It also modulates vitamin D metabolism, negatively regulates osteoblast differentiation and matrix mineralization, and reduces parathyroid hormone secretion from the parathyroid glands. FGF-23 Protein, Rat (His) is the recombinant rat-derived FGF-23 protein, expressed by E. coli , with N-6*His labeled tag. The total length of FGF-23 Protein, Rat (His) is 227 a.a., with molecular weight of ~29.5 kDa.

FGF-23 蛋白是磷酸盐稳态的关键调节剂，通过降低 SLC34A1 水平抑制肾小管磷酸盐转运来发挥其作用。此外，它还具有调节维生素 D 代谢的作用。此外，FGF-23 蛋白负向调节成骨细胞分化和基质矿化。它直接影响甲状旁腺，减少甲状旁腺激素的分泌。在 KL 存在的情况下，FGF-23 蛋白还会上调 EGR1 表达。此外，它与 FGFR1、FGFR2、FGFR3 和 FGFR4 相互作用，并且作为辅助受体的 KL 和硫酸乙酰肝素糖胺聚糖增强成纤维细胞生长因子 (FGF) 与其受体之间的亲和力。

Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells. The ED₅₀ for this effect is 27-51.77 ng/mL in the presence of 1 μg/mL murine Klotho and 100μg/mL heparin.

Rat

E. coli

N-6*His

Q8VI82 (Y25-V251)

170583  [NCBI]

YSDTSPLLGSNWGSLTHLYTATARNSYHLQIHRDGHVDGTPHQTIYSALMITSEDAGSVVIIGAMTRRFLCMDLRGNIFGSYHFSPENCRFRQWTLENGYDVYLSPKHHYLVSLGRSKRIFQPGTNPPPFSQFLARRNEVPLLHFYTARPRRHTRSAEDPPERDPLNVLKPRPRATPIPVSCSRELPSAEEGGPAASDPLGVLRRGRGDARRGAGGTDRCRPFPRFV

Approximately 29.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.