FGL1 蛋白, Human (HEK293, mFc)

FGL1 protein is the main ligand of LAG3 and can inhibit antigen-specific T cell activation, mediating the inhibitory function of LAG3 independently of MHC-II binding. FGL1 is secreted by liver cells and contributes to their growth. FGL1 Protein, Human (HEK293, mFc) is the recombinant human-derived FGL1 protein, expressed by HEK293 , with C-mFc labeled tag.

FGL1 蛋白作为一种免疫抑制分子，通过作为 LAG3 的主要配体对抗原特异性 T 细胞激活发挥抑制作用。它在独立于 MHC II 类 (MHC-II) 结合介导 LAG3 的 T 细胞抑制功能中发挥着关键作用。除了其免疫调节作用外，FGL1 还由肝细胞分泌，有助于其生长。FGL1 作为同二聚体存在，通过其纤维蛋白原 C 末端结构域与 LAG3 相互作用，特异性结合 LAG3 的 Ig 样结构域 1 和 2。研究中详细介绍了这种分子相互作用，强调了 FGL1 在调节免疫反应和肝细胞生长中的重要性，强调了其在 T 细胞激活和肝功能调节中发挥关键作用的潜力。

Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 (HY-P70723) is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Human FGL1.The ED₅₀ of Recombinant Human FGL1-mFc is <2 μg/mL.

Human

HEK293

C-mFc

Q08830 (L23-I312)

2267  [NCBI]

LEDCAQEQMRLRAQVRLLETRVKQQQVKIKQLLQENEVQFLDKGDENTVIDLGSKRQYADCSEIFNDGYKLSGFYKIKPLQSPAEFSVYCDMSDGGGWTVIQRRSDGSENFNRGWKDYENGFGNFVQKHGEYWLGNKNLHFLTTQEDYTLKIDLADFEKNSRYAQYKNFKVGDEKNFYELNIGEYSGTAGDSLAGNFHPEVQWWASHQRMKFSTWDRDHDNYEGNCAEEDQSGWWFNRCHSANLNGVYYSGPYTAKTDNGIVWYTWHGWWYSLKSVVMKIRPNDFIPNVI

Approximately 58-68 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of PBS, 5% Thehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.