GFP 蛋白, Aequorea victoria (His)

The green fluorescent protein (GFP), Aequorea victoria is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range, found in organisms including Aequorea victoria, corals, sea anemones, zoanithids, copepods and lancelets. GFP Protein has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm, while its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP Protein acts as an energy transfer receptor and actively transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer, which is used as expression of reporter genes. GFP Protein, Aequorea victoria (His) is a recombinant jellyfish Aequorea victoria GFP protein with N-6*His labeled tag that consists of 238 amino acids, which is expressed in E. coli ^{[1][2][3][4][5][6][7]}.

GFP 荧光在 Aequorea victoria 中 aequorin 与 Ca²⁺ 离子相互作用时发生，从而导致产生蓝光。部分的发光能量被转移到 GFP 上，使整体颜色向绿色偏移，从而使得 GFP 蛋白能够在活生物中可视化特定蛋白质和细胞结构
^[3]。
GFP 蛋白具有一个 β 桶结构，由十一条 β 链构成，呈褶皱的片状排列，中心有一个含有共价键合色素 4-(p-羟基苯亚甲基) 咪唑烷-5-酮 (HBI) 的 α 螺旋^[4]。
在活细胞成像实验中，一方面，GFP 蛋白直接附着在目标蛋白上，以表明目标基因的成功转染。另一方面，含有突变的从绿色逐渐变为黄色的 GFP 蛋白用于研究蛋白质生产的状态，例如最近激活，持续激活或最近失活^[6]。
GFP 蛋白在癌症研究中广泛用于标记和追踪癌细胞。GFP 蛋白标记的癌细胞被用于模拟转移过程，即癌细胞扩散到远处器官的过程^[7]。

GFP 蛋白 (Aequorea victoria) 是污染物的标记物。当污染物水平增加时，GFP 蛋白和细胞密度都减少，这表明细胞活力有所下降^[5]。

注射了 GFP 蛋白 (Aequorea victoria) 的斑马鱼对细胞压力的识别能力大约是未注射 GFP 蛋白 (Aequorea victoria) 的二十倍^[5]。
肿瘤扩散和转移在双转基因 MMTV-PyMT 和 β-actin 增强型绿色荧光蛋白 (GFP) 小鼠的肿瘤进展过程中发生在不同的时间^[7]。

Prepare a 150 μL recombinant GFP with various concentrations (0.00074 nM - 6.21 μM) in assay buffer and equilibrate to 25°C. Read at excitation wavelengths 485 nm and emission 535 nm.

Others

E. coli

N-6*His

P42212 (M1-K238)

/

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Approximately 27-31 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 1 year, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Prendergast FG, et al. Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea. Biochemistry. 1978 Aug 22;17(17):3448-53.  [Content Brief]

  [2]. Phillips GJ. Green fluorescent protein-a bright idea for the study of bacterial protein localization. FEMS Microbiol Lett. 2001 Oct 16;204(1):9-18.  [Content Brief]

  [3]. Morise H, et al. Intermolecular energy transfer in the bioluminescent system of Aequorea. Biochemistry. 1974 Jun 4;13(12):2656-62.  [Content Brief]

  [4]. Ormö M, et al. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 1996 Sep 6;273(5280):1392-5.  [Content Brief]

  [5]. Song Y H, et al. Noninvasive monitoring of environmental toxicity through green fluorescent protein expressing Escherichia coli[J]. Korean Journal of Chemical Engineering, 2016, 33: 1331-1336.

  [6]. Terskikh A, et al. "Fluorescent timer": protein that changes color with time. Science. 2000 Nov 24;290(5496):1585-8.  [Content Brief]

  [7]. Kouros-Mehr H, et al. GATA-3 links tumor differentiation and dissemination in a luminal breast cancer model. Cancer Cell. 2008 Feb;13(2):141-52.  [Content Brief]