HE4/WFDC2 蛋白, Human (HEK293, His)

The HE4/WFDC2 protein is a homotrimeric broad-spectrum protease inhibitor that regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stress. As a brainstem-restricted receptor, it binds to GDF15 and interacts with RET, activating MAPK and AKT signaling pathways. HE4/WFDC2 Protein, Human (HEK293, His) is the recombinant human-derived HE4/WFDC2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of HE4/WFDC2 Protein, Human (HEK293, His) is 94 a.a., with molecular weight of 18-22 kDa.

HE4/WFDC2 蛋白是一种广泛的蛋白酶抑制剂，通过二硫键形成同源三聚体。它参与各种生理过程，包括调节食物摄入、能量消耗和体重以应对代谢和毒素引起的压力。该蛋白作为脑干限制性受体并与其配体 GDF15 相互作用。结合后，HE4/WFDC2 蛋白与 RET 相互作用并激活 MAPK 和 AKT 信号通路。HE4/WFDC2 蛋白的胞外结构域负责与 GDF15 和 RET 相互作用，作为 GDF15 的受体，并在 GDF15 结合后通过与 RET 的相互作用介导细胞信号传导。值得注意的是，与 RET 的相互作用取决于 GDF15 的先前结合。

Human

HEK293

C-6*His

Q14508 (E31-F124)

10406  [NCBI]

EKTGVCPELQADQNCTQECVSDSECADNLKCCSAGCATFCSLPNDKEGSCPQVNINFPQLGLCRDQCQVDSQCPGQMKCCRNGCGKVSCVTPNF

18-22 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.