IL-36 alpha/IL-1F6 蛋白, Human (153a.a)

IL-36 alpha (IL-1F6), a subform of IL-36 family, belongs to IL-1 superfamily. IL-36 alpha mediates inflammatory response. IL-36 alpha binds to IL-36R and activates NF-κB and MAPK signaling pathways, but the activation requires N-terminal cleavage by neutrophil granule-derived proteases^[1]. IL-36 alpha also binds to IL-1Rrp2 and recruit IL-1RAcP. IL-36 alpha activats the MAPK, Erk1/2 and JNK through IL-36R/IL-1RAcP^[2]. IL-36 alpha/IL-1F6 Protein, Human is a recombinant human IL-36 alpha (K6-F158) without any tag, which is produced in E. coli.

IL-36α是IL-36家族的一个亚型，属于IL-1超家族。IL-36 α 在单核细胞、T/B 淋巴细胞、脾脏、骨髓扁桃体、淋巴结和皮肤中表达^[2]。IL-36 α 在受损肾脏中表达上调。IL-36 α 与肾脏病变、肝细胞癌以及一些炎症/免疫性疾病（包括结肠炎和牛皮癣）的发生有关^[3]。
IL-36 α 在不同物种中存在差异。人 IL-36 α 与小鼠的氨基酸序列同一性<55%。
IL-36α与IL-36R结合并激活NF-κB和MAPK信号通路，从而介导炎症反应。但激活需要中性粒细胞颗粒衍生的蛋白酶（例如组织蛋白酶 G、弹性蛋白酶和蛋白酶-3^[1]）进行 N 末端裂解。IL-36 α 还可以结合 IL-1Rrp2 并募集 IL-1RAcP。IL-36 α 通过 IL-36R/IL-1RAcP^[2] 激活 MAPK、Erk1/2 和 JNK。
IL-36 α是一种促炎因子。IL-36 α 通过激活 NF-κB 和 MAPK 信号通路介导炎症反应^[1]。

IL-36 alpha (human) (10 ng/mL, 48 h) stimulates the production of TNF-α and IL-1β in HCECs^[4].
IL-36 alpha (human) (500 ng/mL, 48 h) induces maturation of human MDDCs^[5].
IL-36 alpha (human) inhibits proliferation, migration, and invasion of both OV2008 and SKOV-3 cells^[6].

Measured by its ability to induce IL-8 secretion by A431 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 21.61 ng/mL. Corresponding to a specific activity is 4.63×10⁴ U/mg.

Human

E. coli

Tag Free

Q9UHA7 (K6-F158)

27179  [NCBI]

KIDTPQQGSIQDINHRVWVLQDQTLIAVPRKDRMSPVTIALISCRHVETLEKDRGNPIYLGLNGLNLCLMCAKVGDQPTLQLKEKDIMDLYNQPEPVKSFLFYHSQSGRNSTFESVAFPGWFIAVSSEGGCPLILTQELGKANTTDFGLTMLF

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 0.02% Tween 20, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.

• [1]. Zhou L,et al. Interleukin-36: Structure, Signaling and Function. Adv Exp Med Biol. 2021;21:191-210.  [Content Brief]

  [2]. Gresnigt MS, et al. Biology of IL-36 cytokines and their role in disease. Semin Immunol. 2013 Dec 15;25(6):458-65.  [Content Brief]

  [3]. Murrieta-Coxca JM, et al. IL-36 Cytokines: Regulators of Inflammatory Responses and Their Emerging Role in Immunology of Reproduction. Int J Mol Sci. 2019 Apr 3;20(7):1649.  [Content Brief]

  [4]. Li JM, et al. IL-36α/IL-36RA/IL-38 signaling mediates inflammation and barrier disruption in human corneal epithelial cells under hyperosmotic stress. Ocul Surf. 2021 Oct;22:163-171.  [Content Brief]

  [5]. Higgins J, et al. IL-36α induces maturation of Th1-inducing human MDDC and synergises with IFN-γ to induce high surface expression of CD14 and CD11c. Hum Immunol. 2015 Apr;76(4):245-53.  [Content Brief]

  [6]. Chang L, et al. IL-36α suppresses proliferation of ovarian cancer cells. Tumour Biol. 2017 Jun;39(6):1010428317706918.  [Content Brief]