IL-4 蛋白, Mouse (CHO)

IL-4 Protein, Mouse (CHO) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes.

小鼠白细胞介素 4 是一种 20 kDa 糖蛋白，由活化的 T 淋巴细胞和肥大细胞合成，调节免疫系统广泛靶细胞的生长和/或分化，包括 B 和 T 淋巴细胞、巨噬细胞和造血祖细胞细胞。鼠白细胞介素 4 (IL-4) 是免疫反应的有效介质，影响造血谱系中多种细胞的生长和分化。该细胞因子由活化的 T 淋巴细胞和肥大细胞表达为 20 kDa 糖蛋白。IL-4 的 cDNA 最初由两个实验室使用表达载体并筛选 IgG 诱导因子或肥大细胞生长因子来分离。从 cDNA 克隆衍生的氨基酸序列用于预测 14 kDa 的 IL-4 的蛋白质主链。这与天然 IL-4 的 N-聚糖酶处理去除 N-连接碳水化合物产生 14 kDa 的蛋白质核心的观察结果一致。使用去糖基化的天然 IL-4 和去糖基化的重组 IL-4（最初在酵母中作为异质的高糖基化分子表达）进行的初步实验表明，IL-4 的碳水化合物修饰不会影响其与受体结合以及刺激 T 和 T 的能力。B 细胞生长^[1]。

The ED₅₀ is <2 ng/mL as measured by murine HT-2 cells, corresponding to a specific activity of >5 × 10⁵ units/mg.

Mouse

CHO

Tag Free

P07750 (H21-S140)

16189  [NCBI]

HIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS

Approximately 15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Levine AD, et al. High level expression and refolding of mouse interleukin 4 synthesized in Escherichia coli. J Biol Chem. 1995 Mar 31;270(13):7445-52.  [Content Brief]