SDF-1 alpha/CXCL12 蛋白, Rat

SDF-1 alpha (Stromal Cell-Derived Factor-1α, SDF-1α) is a member of the chemokine α subfamily that lack the ELR domain. SDF-1α works as a chemoattractant for T- and B-lymphocytes and monocytes. SDF-1α is a ligand for CXCR4. The SDF-1α/CXCR4 signaling mediates many physiological processes including cell trafficking, angiogenesis, embryogenesis, tumor invasion and metastatic. It also controls the chemotaxis of hematopoietic stem cells homing to the bone marrow^[1][2]. SDF-1 alpha/CXCL12 Protein, Rat is produced in E. coli.

基质细胞衍生因子-1（SDF-1）是趋化因子家族的重要成员，有两种亚型：SDF-1α和SDF-1β，其中SDF-1α是主要亚型。SDF-1α广泛存在于人体的许多组织和器官中，如淋巴结、骨髓、肝、肺、肌肉、小肠、肾、脑等，并能在这些器官和组织中持续存在。研究表明，SDF-1α在多种细胞的迁移、分布、发育、分化、凋亡等生理功能中发挥着重要作用。而且，SDF-1α在炎症、肿瘤形成和转移、病原体感染、伤口修复等一些疾病的病理过程中发挥着关键作用^[1][3].
SDF-1 具有 α、β 和 γ 三种亚型，它们在剪接水平上有所不同，而不是在转录水平上有所不同。对人和小鼠 SDF-1 基因组结构的分析揭示了两种亚型：SDF-1α 和 SDF-1β，它们由单个基因编码并由选择性剪接产生。SDF-1α包含3个外显子，编码89个氨基酸的蛋白质，而SDF-1β包含4个外显子，编码93个氨基酸的蛋白质。两种异构体的序列高度相似，唯一的区别是 SDF1β C 末端多了 4 个氨基酸。在成年大鼠大脑中，SDF-1α 是主要的一种，存在于星形胶质细胞、小胶质细胞以及神经元中。SDF-1α 在正常胆碱能神经元（例如内侧隔膜和无名质）和多巴胺能神经元（例如黑质（SN）致密部和腹侧被盖区）中呈阳性。SDF-1α 是 CXCR4 唯一已知的配体。CXCR4 也是人类免疫缺陷病毒 (HIV) 结合的靶点^[1][2]。
使用缺血再灌注模型和模型进行体外和体内研究SDF-1α 预处理可通过激活 ERK-1/2 和 AKT 磷酸化来缩小梗塞面积并增强对缺氧损伤和细胞凋亡的抵抗力^[1]。SDF-1α/CXCR4 信号传导通过与神经递质和神经肽系统、神经内分泌系统的相互作用维持中枢神经系统稳态^[2]。越来越多的动物实验表明，SDF-1α可以增强BMSC的迁移能力，动员BMSC到病变部位，促进其增殖和分化^[3]。

Recombinant rat SDF-1α (0, 5, 10, 30, 50 or 100 ng/mL) activates PI-3K/Akt signaling, and consequently promotes the proliferation and chemotaxis in primary rat vascular smooth muscle cells (VSMCs) ^[4].

Recombinant rat SDF-1α (4 μg/4 μL; intracranial injection) ameliorates TBI-induced brain edema and blood-brain barrier disruption in Sprague-Dawley rats underwent traumatic brain injury (TBI) model. SDF-1α repressed inflammatory responses by inhibiting the expression of pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6. SDF-1α attenuated the brain lesion by affecting the ERK and NF-κB signaling pathways after mechanical head trauma in rats^[5].

1. Full biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.
2. Measured by its ability to inhibit proliferation of Jurkat cells. The ED₅₀ this effect is 3.323 ng/mL, corresponding to a specific activity is 3.01×10⁵ units/mg.
3. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is ≤71.82 ng/mL, corresponding to a specific activity is ≥1.392×10⁴ U/mg.

Rat

E. coli

Tag Free

Q9QZD1/Q80YV8 (K22-K89)

24772  [NCBI]

KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKSNNRQVCIDPKLKWIQEYLDKALNK

Approximately 8-10.07 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4 or PBS, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Santhosh K Ghadge, et al. SDF-1α as a therapeutic stem cell homing factor in myocardial infarction. Pharmacol Ther. 2011 Jan;129(1):97-108.  [Content Brief]

  [2]. Kryczek I, et al. Stroma-derived factor (SDF-1/CXCL12) and human tumor pathogenesis. Am J Physiol Cell Physiol. 2007 Mar;292(3):C987-95.  [Content Brief]

  [3]. Zheng Jiang, et al. Contribution of SDF-1α/CXCR4 signaling to brain development and glioma progression. Neurosignals. 2013;21(3-4):240-58.  [Content Brief]

  [4]. Zhiqiang Meng, et al. SDF Factor-1α Promotes the Migration, Proliferation, and Osteogenic Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells Through the Wnt/β-Catenin Pathway. Stem Cells Dev. 2021 Jan 15;30(2):106-117.  [Content Brief]

  [5]. Wei Jie, et al. SDF-1α/CXCR4 axis is involved in glucose-potentiated proliferation and chemotaxis in rat vascular smooth muscle cells. Int J Exp Pathol. 2010 Oct;91(5):436-44.  [Content Brief]

  [6]. Weifeng Sun, et al. Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats. Inflamm Res. 2014 Apr;63(4):287-97.  [Content Brief]