Spondin-2/SPON2 蛋白, Human (HEK293, His)

Spondin-2 protein is a matricellular protein with antigen binding, lipopolysaccharide binding and metal ion binding activity. Spondin-2 is essential for recruiting lymphocytes and initiating immune responses while being involved in cell adhesion, defense response to other organism; opsonization; and positive regulation of cytokine production. Spondin-2 is found to promote infiltration of M1-like macrophages and inhibits tumor metastasis in cancer research. Spondin-2/SPON2 Protein, Human (HEK293, His) is the recombinant human-derived Spondin-2/SPON2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Spondin-2/SPON2 Protein, Human (HEK293, His) is 305 a.a., with molecular weight of 38-42 kDa.

Spondin-2 蛋白是一种基质细胞蛋白，属于 Mindin F-Spondin 家族成员，具有抗原结合、脂多糖结合和金属离子结合活性。Spondin-2 是一种独特的病原微生物模式识别分子，在募集淋巴细胞和启动免疫应答中发挥重要作用。Spondin-2 参与细胞黏附，并在多个生物过程的上游或内部发挥作用，包括对其他生物的防御反应、调理素作用、以及正向调节细胞因子产生。Spondin-2 也作为炎症细胞募集和 T 细胞激发的整合素配体发挥作用。Spondin-2 与细菌的结合促进细菌的吞噬并刺激巨噬细胞产生促炎细胞因子。在肿瘤研究中，Spondin-2 被发现促进 M1 样巨噬细胞的浸润并抑制肿瘤转移^[1][2][3]。

Measured by the ability to enhance BMP-2 induced alkaline phosphatase activity in MC3T3-E1 cells. The ED₅₀ for this effect is approximately 0.949 μg/mL in the presence of 0.113 μg/mL of BMP-2, corresponding to a specific activity is 1.054×10³ units/mg.

Human

HEK293

C-6*His

AAH02707.1 (Q27-V331)

10417  [NCBI]

QPLGGESICSARAPAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHEVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLLRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

39-47 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM EDTA, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.