TGF beta 1/TGFB1 蛋白, Canine (HEK293, His)

TGFB1 proprotein is the precursor of latency-associated peptide (LAP) and active transforming growth factor Beta-1 (TGF-β-1) chain, which maintains TGF-β-1 latency in the extracellular matrix. TGFB1 binds non-covalently to TGF-β-1 and interacts with “environmental molecules” (LTBP1, LRRC32/GARP, LRRC33/NRROS) to regulate TGF-β-1 activation. TGF beta 1/TGFB1 Protein, Canine (HEK293, His) is the recombinant canine-derived TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag. The total length of TGF beta 1/TGFB1 Protein, Canine (HEK293, His) is 361 a.a..

转化生长因子 Beta-1 (TGFB1) 前蛋白是潜伏相关肽 (LAP) 和活性转化生长因子 Beta-1 (TGF-beta-1) 链的前体，构成调节亚基和活性亚基分别为 TGF-β-1。它在细胞外基质内储存期间维持 TGF-β-1 链处于潜在状态方面发挥着至关重要的作用。TGFB1 与 TGF-β-1 非共价结合，并通过与“环境分子”（如 LTBP1、LRRC32/GARP 和 LRRC33/NRROS）相互作用来调节其激活，从而控制 TGF-β-1 的激活。值得注意的是，与 LRRC33/NRROS 的相互作用调节巨噬细胞和小胶质细胞中 TGF-β-1 的激活，而与 LRRC32/GARP 的相互作用控制激活的调节性 T 细胞 (Treg) 表面的激活。此外，TGFB1 与整合素（ITGAV:ITGB6 或 ITGAV:ITGB8）的相互作用会诱导潜伏期相关肽链的扭曲，导致随后释放活性 TGF-β-1。

Measured by its binding ability in a functional ELISA. Immobilized Canine TGFB1 at 5 μg/ml (100μl/well) can bind biotinylated LRRC32. The ED₅₀ for this effect is 0.433 μg/mL.

Canine

HEK293

C-6*His

P54831 (L30-S390)

403998  [NCBI]

LSTCKTIDMELVKRKRIEAIRGQILSKLRLSSPPSQGEVPPVPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVENTNKIYEKVKKSPHSIYMLFNTSELREAVPEPVLLSRAELRLLRLKLKAEQHVELYQKYSNDSWRYLSNRLLAPSDTPEWLSFDVTGVVRQWLSHGGEVEGFRLSAHCSCDSKDNTLQVDINGFSSSRRGDLATIHGMNRPFLLLMATPLERAQHLHSSRQRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 53-60 & 38-45 & 15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.