TGF beta 1/TGFB1 蛋白, Human (112a.a, HEK293)

TGF beta 1/TGFB1 Protein is initially identified as a growth factor that induces the growth of rodent fibroblasts. TGF beta 1/TGFB1 Protein inhibits the cell cycle in the G1 phase. TGF beta 1/TGFB1 is an endogenous factor controlling apoptosis in normal and pathological tissues. TGF beta 1/TGFB1 Protein, Human (112a.a, HEK293) is a recombinant protein (A279-S390) produced by HEK293 cells^[1][2].

TGFβ由脱颗粒血小板释放，并由参与修复过程的所有主要细胞类型分泌，包括淋巴细胞、巨噬细胞、内皮细胞、平滑肌细胞、上皮细胞和成纤维细胞。哺乳动物表达 TGFβ 的三种亚型，分别称为 TGFβI、TGFβ2 和 TGFβ3；TGFβ1 是所有组织中最丰富的异构体，并且在人类血小板中，它是该肽的唯一异构体。某些细胞如视网膜色素上皮细胞主要分泌TGFβ2，某些体液如眼房水和玻璃体、羊水、唾液和母乳主要含有TGFβ2。TGFβ3 是 TGFβ 亚型中研究最少的。它是从人脐带中分离出来的，由某些细胞分泌，包括成肌细胞系；然而，它在组织和细胞提取物中的含量通常低于 TGFβ1 或 TGFβ2^[1]。其活性有三个基本方向：一、TGFβ1调节细胞增殖、生长、分化和细胞运动。二. TGFβ1具有免疫调节作用。三． TGFβ1 具有促纤维化作用。TGFβ1 的作用可以是局部的和全身的^[2]。

1.Immobilized Human Mature TGF beta 1, No Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC₅₀ of <8 ng/mL determined by ELISA.
2.Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED₅₀ for this effect is 0.06323-0.0964 ng/mL, corresponding to a specific activity is 1.037-1.58×10⁷ units/mg.

• 
  Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED₅₀ for this effect is 0.0964 ng/mL, corresponding to a specific activity is 1.037×10⁷ units/mg.

Human

HEK293

Tag Free

P01137 (A279-S390)

7040  [NCBI]

ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 13-15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50mM Glycine 150mM NaCl, pH 2.5 or 4mM HCL pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4mM HCl.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. K. Miyazono, et al. A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1. The EMBO Journal (1991)10:1091-1101.

  [2]. M Selvakumaran, et al. The novel primary response gene MyD118 and the proto-oncogenes myb, myc, and bcl-2 modulate transforming growth factor beta 1-induced apoptosis of myeloid leukemia cells. Mol Cell Biol. 1994 Apr;14(4):2352-60.  [Content Brief]

  [3]. Chambaz E.M., et al. Transforming Growth Factor-βs: A Multifunctional Cytokine Family. Implication in the Regulation of Adrenocortical Cell Endocrine Functions. 1991.

  [4]. Dulce Maroni, et al. TGFB1 disrupts the angiogenic potential of microvascular endothelial cells of the corpus luteum. Cell Sci. 2011 Jul 15;124(Pt 14):2501-10.  [Content Brief]

  [5]. Nan Wu, et al. LINC00941 promotes CRC metastasis through preventing SMAD4 protein degradation and activating the TGF-β/SMAD2/3 signaling pathway. Cell Death Differ. 2021 Jan;28(1):219-232.  [Content Brief]

  [6]. Hai Zhang, et al. Effects of transforming growth factor-beta 1 (TGF-beta1) on in vitro mineralization of human osteoblasts on implant materials. Biomaterials. 2003 May;24(12):2013-20.  [Content Brief]

  [7]. Paloma B Liton, et al. Cross-talk between TGF-beta1 and IL-6 in human trabecular meshwork cells. Mol Vis. 2009;15:326-34. Epub 2009 Feb 11.  [Content Brief]