TGF beta 1/TGFB1 LAP 蛋白, Human (HEK293, His)

TGF beta 1/TGFB1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. TGF beta 1 is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, apoptosis, and can regulate the expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. TGF beta 1/TGFB1 LAP Protein, Human (HEK293, His) is the recombinant human-derived TGF beta 1/TGFB1 Latency-associated peptide protein, expressed by HEK293 , with C-10*His labeled tag and C33S mutation.

转化生长因子 (TGF) β 1 是细胞因子转化生长因子 β 超家族的多肽成员。TGF beta 1 是一种分泌蛋白，具有多种细胞功能，包括控制细胞生长、细胞增殖、细胞分化、凋亡，并且可以调节其他生长因子的表达和激活，包括干扰素 gamma 和肿瘤坏死因子 alpha。在人类中，TGF-β1 由 TGFB1 基因编码。TGF beta 1 通过调节膜内蛋白水解作用激活 CREB3L1，刺激持续的胶原蛋白生成。TGF beta 1 通过诱导 SMAD2/3 磷酸化和随后易位至细胞核来介导 SMAD2/3 激活，并通过促进 IPO7 介导的磷酸化 SMAD2 易位至细胞核和随后靶基因的转录，来调节牙乳头细胞中成牙本质细胞分化。TGF beta 1 可诱导多种细胞类型的上皮间质转化 (EMT) 和细胞迁移。TGF beta 1 在控制免疫系统中发挥重要作用，并且对不同类型的细胞或不同发育阶段的细胞表现出不同的活性 ^{[1][2][3][4][5][6]}。

Measured by its ability to inhibit TGF-beta 1 activity on HT-2 mouse T cells. The ED₅₀ this effect is ≤87.58 ng/mL in the presence of 1 ng/mL of Recombinant Human TGF beta 1, corresponding to a specific activity is ≥1.14×10⁴ units/mg.

Human

HEK293

C-10*His

P01137 (L30-R278, C33S)

7040  [NCBI]

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRR

Approximately 40-57 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.