TGF beta 1/TGFB1 蛋白, Human (CHO)

TGF beta 1/TGFB1 Protein is initially identified as a growth factor that induces the growth of rodent fibroblasts. TGF beta 1/TGFB1 Protein inhibits the cell cycle in the G1 phase. TGF beta 1/TGFB1 is an endogenous factor controlling apoptosis in normal and pathological tissues. TGF beta 1/TGFB1 Protein, Human is a recombinant protein (A279-S390) produced by CHO cells^[1][2].

TGF beta 1/TGFB1 蛋白（转化生长因子 beta 1）是一种多功能细胞因子，几乎由所有细胞合成。TGF beta 1/TGFB1 蛋白具有很强的与 TGFbRII^[3] 结合的能力。
不同物种的TGFb1蛋白的氨基酸序列非常稳定，由此得出结论：TGFb在进化过程中仅发生了轻微改变，无论在人类还是在动物中，其功能都是相似的。TGFβ1/TGFB1 蛋白作为无活性肽分泌，形成“潜在复合物”的一部分，该复合物由非共价结合至其潜伏相关肽 (LAP) 的成熟 TGFB1 二聚体组成，并通过 LAP 与潜在的 TGFB 结合蛋白质（LTBP）。激活的 TGF beta 1/TGFB1 蛋白与普遍表达的细胞表面 TGFB1 I 型受体 (TGFBRI) 和 II 型受体 (TGFBRII) 结合，它们是跨膜丝氨酸/苏氨酸激酶^[4]。
TGF beta 1/TGFB1 蛋白调节细胞增殖、生长、分化和细胞运动。TGFb1具有免疫调节作用。TGF beta 1/TGFB1 蛋白具有促纤维化作用。TGF beta 1/TGFB1 蛋白作用可以是局部的和全身的。TGF beta 1/TGFB1 蛋白在发育、纤维化和癌症中发挥驱动作用^[4]。

TGFB1 human (0.2 ng/mL) upregulates LINC00941^[5].
TGF-β1 (0.2 ng/mL) significantly and maximally inhibits the growth of MLE cells^[6].
TGF–β1 (5 ng/ml) results in a significant increase in the protein and mRNA levels of IL-6 in HTM cells^[7].

1. The ED₅₀ is <0.2 ng/mL as measured in ability to inhibit the mouse IL-4-dependent proliferation of HT-2 cells.
2. The ability to inhibit the IL-4-dependent proliferation of TF 1 human erythroleukemic cells has an ED₅₀ value of 4-40 pg/mL.
3. Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED₅₀ for this effect is ≤0.09738 ng/mL, corresponding to a specific activity is ≥1.027×10⁷ units/mg.

• 
  Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED₅₀ for this effect is 0.09738 ng/mL, corresponding to a specific activity is 1.027×10⁷ units/mg.

Human

CHO

Tag Free

P01137 (A279-S390)

7040  [NCBI]

ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 12-14 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM NaAc, 50 mM NaCl, pH 5.0 or 50 mM Glycine-HCl, 150 mM NaCl, pH 2.5 or 4 mM HCL.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O or diluted with 50 mM Citrate. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. K. Miyazono, et al. A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1. The EMBO Journal (1991)10:1091-1101.

  [2]. M Selvakumaran, et al. The novel primary response gene MyD118 and the proto-oncogenes myb, myc, and bcl-2 modulate transforming growth factor beta 1-induced apoptosis of myeloid leukemia cells. Mol Cell Biol. 1994 Apr;14(4):2352-60.  [Content Brief]

  [3]. Chambaz E.M., et al. Transforming Growth Factor-βs: A Multifunctional Cytokine Family. Implication in the Regulation of Adrenocortical Cell Endocrine Functions. 1991.

  [4]. Dulce Maroni, et al. TGFB1 disrupts the angiogenic potential of microvascular endothelial cells of the corpus luteum. Cell Sci. 2011 Jul 15;124(Pt 14):2501-10.  [Content Brief]

  [5]. Nan Wu, et al. LINC00941 promotes CRC metastasis through preventing SMAD4 protein degradation and activating the TGF-β/SMAD2/3 signaling pathway. Cell Death Differ. 2021 Jan;28(1):219-232.  [Content Brief]

  [6]. Hai Zhang, et al. Effects of transforming growth factor-beta 1 (TGF-beta1) on in vitro mineralization of human osteoblasts on implant materials. Biomaterials. 2003 May;24(12):2013-20.  [Content Brief]

  [7]. Paloma B Liton, et al. Cross-talk between TGF-beta1 and IL-6 in human trabecular meshwork cells. Mol Vis. 2009;15:326-34. Epub 2009 Feb 11.  [Content Brief]