2. Metabolic Enzyme/Protease MAPK/ERK Pathway
  3. Endogenous Metabolite p38 MAPK
  4. 7-Ketocholesterol

7-Ketocholesterol 

目录号: HY-113342 纯度: 99.87%
COA 产品使用指南

摩尔计算器

7-Ketocholesterol 是胆固醇氧化产物，广泛存在于动脉粥样硬化斑块中，比胆固醇具有更强的致动脉粥样硬化作用。7-Ketocholesterol 能够抑制胆汁酸和胆固醇的合成限速酶，如胆固醇 7α-羟化酶和 HMG-CoA 还原酶。7-Ketocholesterol 在体内外具有促炎作用，可诱导细胞凋亡 (apoptosis)。

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7-Ketocholesterol Chemical Structure

7-Ketocholesterol Chemical Structure

CAS No. : 566-28-9

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规格 价格 是否有货 数量
1 mg ￥304
In-stock
5 mg ￥800
In-stock
10 mg ￥1300
In-stock
25 mg ￥2600
In-stock
50 mg ￥3988
In-stock
100 mg ￥5725
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

Other Forms of 7-Ketocholesterol:

7-Ketocholesterol 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 p38 MAPK 亚型特异性产品:

查看所有亚型
p38 MAPK p38α p38β MAPK13 p38δ p38γ

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

7-Ketocholesterol is an oxidation product of cholesterol, widely present in atherosclerotic plaques, and has a stronger atherogenic effect than cholesterol. 7-Ketocholesterol can inhibit the rate-limiting enzymes involved in bile acid and cholesterol synthesis, such as cholesterol 7α-hydroxylase and HMG-CoA reductase. 7-Ketocholesterol exhibits pro-inflammatory effects both in vivo and in vitro and can induce cell apoptosis (apoptosis)[1].

IC50 & Target

Human Endogenous Metabolite

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
ARPE-19 IC50
32.3 μM
Compound: 4
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
C6 IC50
20 μM
Compound: 7KC
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
[PMID: 24211631]
HEK293 EC50
8.7 μM
Compound: 9
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
[PMID: 24928400]
HEK293 EC50
9.7 μM
Compound: 6
Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
[PMID: 23830695]
HT-29 IC50
25.7 μM
Compound: 4
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HT-29 IC50
25.7 μM
Compound: 6
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
Vero IC50
75 μM
Compound: 1
Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay
Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay
[PMID: 24084159]
体外研究
(In Vitro)

7-Ketocholesterol (50 μM，2.5-2 小时) 可通过 p38 MAPK 通路增强白细胞与内皮细胞的粘附[4]。
7-Ketocholesterol (2.5-40 μg/mL，24 小时) 以剂量依赖性方式激活人微血管内皮细胞中的 caspase-3/7、-8 和 -12，从而诱导细胞凋亡[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

7-Ketocholesterol 相关抗体:

Western Blot Analysis[4]

Cell Line: Human umbilical vein endothelial cells (HUVECs)
Concentration: 50 μM
Incubation Time: 0.5-2 h
Result: Stimulated the phosphorylation of p38 MAPK, reaching a peak within 30-120 minutes, with no significant effect on JNK.
Enhanced the adhesion of THP-1 cells to HUVECs and upregulated the expression of E-selectin.
Promoted the expression of E-selectin by activating the transcription factor ATF-2.

Cell Viability Assay[5]

Cell Line: Human umbilical vein endothelial cells (HUVECs)
Concentration: 2.5, 5, 10, 15, 20, 40 μg/mL
Incubation Time: 24 h
Result: The average cell viability of HMVEC cells treated with 7-Ketocholesterol at concentrations of 40, 20, 10, 5, and 2.5 μg/ml for 24 hours was 45.33%, 72.83% (p < 0.001), 82.7% (p < 0.05), 88.47%, and 88.5% (p > 0.05), respectively, while the untreated control was 88.83%.
体内研究
(In Vivo)

7-Ketocholesterol (5-20 mg/mL，通过生物降解晶片植入大鼠眼前房，单次给药) 在棕色挪威大鼠体内具有促血管生成和促炎作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Brown Norway rats (150-200 g)[3]
Dosage: 5, 10, 15, 20 mg/mL
Administration: Implanting biodegradable wafers containing 7-Ketocholesterol into the anterior chamber of rats' eyes, single dose
Result: Significantly increased angiogenesis, with a large infiltration of macrophages detected by immunohistochemistry.
Promoted the growth of new blood vessels originating from the limbus and extending through the cornea, peaking between days 7 and 10 after implantation.
Led to a marked increase in vascular endothelial growth factor (VEGF), IL-1b, and GRO/KC in the aqueous humor.
分子量

400.64

Formula

C27H44O2
CAS 号
性状

固体

颜色

White to off-white

结构分类
初始来源

human
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 6.25 mg/mL (15.60 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4960 mL 12.4800 mL 24.9601 mL
5 mM 0.4992 mL 2.4960 mL 4.9920 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.87%

COA (286 KB) HNMR (262 KB) RP-HPLC (1247 KB) LCMS (133 KB)

产品使用指南 (1538 KB)
参考文献

7-Ketocholesterol 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4960 mL 12.4800 mL 24.9601 mL 62.4002 mL
5 mM 0.4992 mL 2.4960 mL 4.9920 mL 12.4800 mL
10 mM 0.2496 mL 1.2480 mL 2.4960 mL 6.2400 mL
15 mM 0.1664 mL 0.8320 mL 1.6640 mL 4.1600 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Ketocholesterol566-28-9Endogenous Metabolitep38 MAPKHMG-CoA reductasecholesterol biosynthesisorphan diseasecoronary artery diseaseInhibitorinhibitorinhibit

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目录号：
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