2. Cell Cycle/DNA Damage
  3. Antifolate
  4. Aminopterin

Aminopterin  (Synonyms: 氨基蝶呤; 4-Aminofolic acid; APGA)

目录号: HY-14518 纯度: ≥98.0%
COA 产品使用指南

摩尔计算器

Aminopterin (4-Aminofolic acid)，叶酸的 4-氨基衍生物，是叶酸 (folic acid) 拮抗剂。Aminopterin 催化叶酸还原为四氢叶酸，Aminopterin 竞争性抑制二氢叶酸还原酶 (DHFR)，Ki 值为 3.7 pM。Aminopterin 具有抗癌和免疫抑制活性。Aminopterin 用于研究儿童白血病。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Aminopterin Chemical Structure

Aminopterin Chemical Structure

CAS No. : 54-62-6

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥550
In-stock
5 mg ￥500
In-stock
10 mg ￥800
In-stock
25 mg ￥1440
In-stock
50 mg ￥2160
In-stock
100 mg ￥3240
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Aminopterin:

Aminopterin 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Aminopterin (4-Aminofolic acid), the 4-amino derivative of folic acid, is a folic acid antagonist. Aminopterin catalyses the reduction of folic acid to tetrahydrofolic acid, and competitively inhibits dihydrofolate reductase (DHFR) with a Ki of 3.7 pM. Aminopterin has anticancer and immunosuppressive activity. Aminopterin is used in treatment of pediatric leukemia[1][2].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
CCRF-CEM IC50
3 nM
Compound: AMT
Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target
Tested in vitro for inhibitory concentration against CCRF-CEM human Leukemic lymphoblast by using DHFR as primary target
[PMID: 8035423]
CCRF-CEM IC50
3 μM
Compound: AMT
Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM)
Inhibitory activity against uptake of [3H]MTX by human leukemia cells (CCRF-CEM)
[PMID: 8568828]
HeLa IC50
3.6 nM
Compound: AMT
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay
[PMID: 31843461]
HeLa IC50
8.3 nM
Compound: AMT
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay
[PMID: 31843461]
HL-60 IC50
2.5 nM
Compound: AMT
Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay
Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs in absence of ROS scavenger pyruvate by Alamar blue assay
[PMID: 31843461]
HL-60 IC50
3 nM
Compound: AMT
Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay
Cytotoxicity against human HL60 cells assessed reduction in cell viability incubated for 48 hrs in presence of ROS scavenger pyruvate by Alamar blue assay
[PMID: 31843461]
L1210 IC50
0.002 μM
Compound: 2
Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure.
Ability to inhibit L1210 murine leukemia tumor cell growth in culture after 48 hr of its exposure.
[PMID: 1992118]
体外研究
(In Vitro)

The IC50 value of Aminopterin (4-Aminofolic acid) against CCRF-CEM cells during 72 h of exposure is 4.4 nM[2].
Aminopterin (4-Aminofolic acid) produces a marked inhibition of mitosis in low concentrations in human leukemic leukocytes[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Aminopterin 相关抗体:
Clinical Trial
分子量

440.41

Formula

C19H20N8O5
CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

甲氨蝶呤EP杂质B
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 14.71 mg/mL (33.40 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : 5 mg/mL (11.35 mM; ultrasonic and adjust pH to 8 with NaOH)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.2706 mL 11.3531 mL 22.7061 mL
5 mM 0.4541 mL 2.2706 mL 4.5412 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: ≥98.0%

COA (192 KB) HNMR (278 KB)

产品使用指南 (1538 KB)
参考文献

Aminopterin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.2706 mL 11.3531 mL 22.7061 mL 56.7653 mL
5 mM 0.4541 mL 2.2706 mL 4.5412 mL 11.3531 mL
10 mM 0.2271 mL 1.1353 mL 2.2706 mL 5.6765 mL
DMSO 15 mM 0.1514 mL 0.7569 mL 1.5137 mL 3.7844 mL
20 mM 0.1135 mL 0.5677 mL 1.1353 mL 2.8383 mL
25 mM 0.0908 mL 0.4541 mL 0.9082 mL 2.2706 mL
30 mM 0.0757 mL 0.3784 mL 0.7569 mL 1.8922 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Aminopterin54-62-64-Aminofolic acid APGA甲氨蝶呤EP杂质BAntifolateleukemicleukocytesfolicacidtetrahydrofolicanticancerimmunosuppressiveInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Aminopterin
目录号：
HY-14518
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z